Mouse Orexin (OX) ELISA Kit | OX elisa kit

Mouse Orexin (OX) ELISA Kit

Cat. Num. AAA2700080

• Gene Names: HCRT; OX; PPOX; NRCLP1
• Reactivity: Mouse
• Synonyms: Orexin (OX); Mouse Orexin (OX) ELISA Kit; HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor; OX elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Orexin (OX).; No significant cross-reactivity or interference between Orexin (OX) and analogues was observed.
• Sequence Length: 131

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 6.17-500pg/mL
• Sensitivity: < 2.65pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Orexin (OX) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Orexin (OX) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OX elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Orexin (OX) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Orexin (OX) and unlabeled Orexin (OX) (Standards or samples) with the pre-coated antibody specific to Orexin (OX). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Orexin (OX) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Orexin (OX) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4557635
• NCBI GeneID: 3060
• NCBI Accession #: NP_001515.1
• NCBI GenBank Nucleotide #: NM_001524.1
• UniProt Accession #: O43612
• Molecular Weight: 13,363 Da
• NCBI Official Full Name: orexin
• NCBI Official Synonym Full Names: hypocretin neuropeptide precursor
• NCBI Official Symbol: HCRT
• NCBI Official Synonym Symbols: OX; PPOX; NRCLP1
• NCBI Protein Information: orexin
• UniProt Protein Name: Orexin
• UniProt Gene Name: HCRT
• UniProt Synonym Gene Names: OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2

NCBI Description

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

Uniprot Description

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.