Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat Stearoyl-CoA desaturase Polyclonal Antibody | anti-SCD antibody
Goat anti-Stearoyl-CoA desaturase Antibody
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml.
Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS421254 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS421254 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Flow Cytometry (FC)
(MBS421254 (2.5ul) staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 in red.)
Flow Cytometry (FC)
(MBS421254 (2.5ul) staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 in red.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]
Uniprot Description
SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family.
Protein type: Endoplasmic reticulum; Membrane protein, integral; EC 1.14.19.1; Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; Oxidoreductase
Chromosomal Location of Human Ortholog: 10q24.31
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane
Molecular Function: iron ion binding; oxidoreductase activity; stearoyl-CoA 9-desaturase activity
Biological Process: unsaturated fatty acid biosynthetic process
Research Articles on SCD
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Product Notes
The SCD scd (Catalog #AAA421254) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Stearoyl-CoA desaturase Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Stearoyl-CoA desaturase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10ug/ml. Western Blot: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml. Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes. Researchers should empirically determine the suitability of the SCD scd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RIKRTGDGNY KSG. It is sometimes possible for the material contained within the vial of "Stearoyl-CoA desaturase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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