Rabbit anti-Human Stathmin 1 Polyclonal Antibody | anti-STMN1 antibody

Stathmin 1 (Oncoprotein 18, STMN1)

Cat. Num. AAA621199

• Gene Names: STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; FLJ32206; MGC138869; MGC138870
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Stathmin 1; Polyclonal Antibody; Stathmin 1 (Oncoprotein 18; STMN1); Anti -Stathmin 1 (Oncoprotein 18; anti-STMN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total Stathmin 1 protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-STMN1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthesized non-phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 37 (P-L-SP-P-P)
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS621199.)

Western Blot (WB)

(Western blot analysis of extract from Jurkat cells untreated or treated with PMA (1 ng/ml, 15 min), using Stathmin 1 (Ab-38) Polyclonal Antibody (lane 1 and 2) and Stathmin 1 (Phospho-Ser38) Polyclonal Antibody (lane 3 and 4))

Related Product Information for anti-STMN1 antibody

Stathmin 1/oncoprotein 18, also known as STMN1, is a highly conserved 17kD protein. Its function as an important regulatory protein of microtubule dynamics has been well characterized.[1] Eukaryotic microtubules are one of three major components of the cell's cytoskeleton. They are highly dynamic structures that continuously alternate between assembly and disassembly. Stathmin performs an important function in regulating rapid microtubule remodeling of the cytoskeleton in response to the cell's needs. Microtubules are cylindrical polymers of a,b-tubulin. Their assembly is in part determined by the concentration of free tubulin in the cytoplasm.[2]

Stathmin interacts with two molecules of dimeric a,b-tubulin to form a tight ternary complex called the T2S complex. [1] One mole of stathmin binds to two moles of tubulin dimers through the stathmin-like domain (SLD).[3] When stathmin sequesters tubulin into the T2S complex, tubulin becomes non-polymerizable. Without tubulin polymerization, there is no microtubule assembly. Through this mechanism, stathmin promotes microtubule disassembly without acting directly on the microtubule ends.[1] The rate of microtubule assembly is an important aspect of cell growth therefore associating regulation of stathmin with cell cycle progress. Regulation of stathmin is cell cycle dependent and controlled by the cell's protein kinases in response to specific cell signals.[3] Phosphorylation at four serine residues on stathmin named Ser16, Ser25, Ser38 and Ser63 causes weakened stathmin-tubulin binding. Stathmin phosphorylation increases the concentration of tubulin available in the cytoplasm for microtubule assembly. For cells to assemble the mitotic spindle necessary for initiation of the mitotic phase of the cell cycle, stathmin phosphorylation must occur. Without microtuble growth and assembly, the mitotic spindle cannot form, and the cell cycle is arrested. At cytokinesis, the last phase of the cell cycle, rapid dephosphorylation of stathmin occurs to block the cell from entering back into the cell cycle until it is ready.[3]
Stathmin's role in regulation of the cell cycle causes it to be an oncoprotein named oncoprotein 18 (op18). As op18, stathmin can cause uncontrolled cell proliferation when mutated and not functioning properly. If stathmin is unable to bind to tubulin, it allows for constant microtubule assembly and therefore constant mitotic spindle assembly. With no regulation of the mitotic spindle, the cell cycle is capable of cycling uncontrollably resulting in the unregulated cell growth characteristic of cancer cells.[3]
Mice without stathmin have deficiency in innate and learned fear. Stathmin negative females do not assess threats well, leading to lack of innate parental care and adult social interactions. They lack motivation for retrieving pups and are unable to choose a safe location for nest-building. However, they have an enhancement in social interactions.[4]

Product Categories/Family for anti-STMN1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 197692597
• NCBI GeneID: 3925
• UniProt Accession #: P16949; A2A2D1; B2R4E7; B7Z8N4; D3DPJ5; Q59G27; Q96CE4
• Molecular Weight: 17,303 Da
• NCBI Official Full Name: stathmin 1
• NCBI Official Synonym Full Names: stathmin 1
• NCBI Official Symbol: STMN1
• NCBI Official Synonym Symbols: Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; FLJ32206; MGC138869; MGC138870
• NCBI Protein Information: stathmin; prosolin; metablastin; oncoprotein 18; phosphoprotein 19; OTTHUMP00000008527; OTTHUMP00000008528; OTTHUMP00000008530; OTTHUMP00000230726; phosphoprotein p19; stathmin 1/oncoprotein 18; transmembrane protein C1orf215; leukemia-associated phosphoprotein p18
• UniProt Protein Name: Stathmin
• Protein Family: Stathmin
• UniProt Gene Name: STMN1
• UniProt Synonym Gene Names: C1orf215; LAP18; OP18
• UniProt Entry Name: STMN1_HUMAN

NCBI Description

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

STMN1: a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Phosphorylation reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, nonleukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: microtubule; neuron projection; membrane; cytoplasm; intracellular; cytosol

Molecular Function: tubulin binding; signal transducer activity

Biological Process: mitotic spindle organization and biogenesis; axonogenesis; response to virus; negative regulation of microtubule polymerization; microtubule depolymerization; positive regulation of cell motility; brain development; signal transduction; neurite development; regulation of cytoskeleton organization and biogenesis