Rabbit anti-Human, Mouse STAT6 Polyclonal Antibody | anti-STAT6 antibody

STAT6, Phosphorylated (Tyr641) (Signal Transducer and Activator of Transcription 6, IL-4 Stat)

Cat. Num. AAA649651

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: STAT6; Polyclonal Antibody; Phosphorylated (Tyr641) (Signal Transducer and Activator of Transcription 6; IL-4 Stat); Anti -STAT6; anti-STAT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Stat6 at ~110kD. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-STAT6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry and Western Blot.; Dilution: Immunohistochemistry: 20ug/ml; Western Blot: 1-4ug/ml
• Immunogen: Synthetic phosphopeptide corresponding to Tyr641 from human Stat6.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-STAT6 antibody

Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3b appears to be activated by both while Stat3a is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Product Categories/Family for anti-STAT6 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 23397678
• NCBI GeneID: 6778
• NCBI Accession #: NP_003144.3
• NCBI GenBank Nucleotide #: NM_003153.4
• UniProt Accession #: P42226; Q5FBW5; Q71UP4; A8K316; B7ZA27; F5GXI9
• Molecular Weight: 94,135 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT
• UniProt Protein Name: Signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: STAT6
• UniProt Entry Name: STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Function: Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. Ref.9

Subunit structure: Forms a homodimer or a heterodimer with a related family member

By similarity. Interacts with NCOA1 via its C-terminal LXXLL motif. Ref.8

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Post-translational modification: Tyrosine phosphorylated following stimulation by IL4/interleukin-4 and IL3/interleukin-3

By similarity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling. Ref.9

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.