Western Blot (WB)
(Western blot detection of STAT5a/b in K562,C6,3T3,Hela cell lysates using STAT5a/b Rabbit mAb(1:1000 diluted). Predicted band size:90kDa. Observed band size:90kDa.)
Rabbit STAT5a/b Monoclonal Antibody | anti-STAT5A antibody
STAT5a/b Rabbit mAb
Gene Names
Stat5a; STAT5; AA959963
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
STAT5a/b; Monoclonal Antibody; STAT5a/b Rabbit mAb; Signal transducer and activator of transcription 5A; MGF; STAT5; anti-STAT5A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R09-5F2
Specificity
In the virgin, found in most tissues except brain and muscle. During lactation, abundantly found in mammary tissue, as well as in other secretory organs such as salivary gland and seminal vesicle. Gene expression databases
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-STAT5A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:200
ICC/IF: 1:50
FC/FACS: 1:20
IP: 1:50
IHC: 1:200
ICC/IF: 1:50
FC/FACS: 1:20
IP: 1:50
Immunogen
Recombinant protein of human STAT5A
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of STAT5a/b in K562,C6,3T3,Hela cell lysates using STAT5a/b Rabbit mAb(1:1000 diluted). Predicted band size:90kDa. Observed band size:90kDa.)
Western Blot (WB)
(Western blot detection of STAT5a/b in K562,C6,3T3,Hela cell lysates using STAT5a/b Rabbit mAb(1:1000 diluted). Predicted band size:90kDa. Observed band size:90kDa.)
Related Product Information for anti-STAT5A antibody
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
90,831 Da
NCBI Official Full Name
Signal transducer and activator of transcription 5A
NCBI Official Synonym Full Names
signal transducer and activator of transcription 5A
NCBI Official Symbol
Stat5a
NCBI Official Synonym Symbols
STAT5; AA959963
NCBI Protein Information
signal transducer and activator of transcription 5A
UniProt Protein Name
Signal transducer and activator of transcription 5A
Protein Family
UniProt Gene Name
Stat5a
UniProt Synonym Gene Names
Mgf; Mpf
Uniprot Description
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Research Articles on STAT5A
Similar Products
Product Notes
The STAT5A stat5a (Catalog #AAA4753042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAT5a/b Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STAT5a/b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:200 ICC/IF: 1:50 FC/FACS: 1:20 IP: 1:50. Researchers should empirically determine the suitability of the STAT5A stat5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5a/b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
