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Testing Data
(Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 12 hours after treatment. The blue staining is DAPI. MBS601235 detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.)
Mouse anti-Mouse, Rat Neuron Specific Nuclear Protein Monoclonal Antibody
Neuron Specific Nuclear Protein (NeuN) (Biotin)
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Neuron Specific Nuclear Protein; Monoclonal Antibody; Neuron Specific Nuclear Protein (NeuN) (Biotin); Anti -Neuron Specific Nuclear Protein (NeuN) (Biotin); anti-Neuron Specific Nuclear Protein antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H412
Specificity
Recognizes mouse Neuron Specific Nuclear Protein (NeuN). Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Non-reactive cell types include Purkinje, mitral and photoreceptor cells. Species Crossreactivity: rat
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.1% sodium azide. Labeled with Biotin.
Applicable Applications for anti-Neuron Specific Nuclear Protein antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.
Dilution: Immunohistochemistry: 1:100; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde- based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.
Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD.
Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100.
Dilution: Immunohistochemistry: 1:100; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde- based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.
Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD.
Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100.
Immunogen
Purified cell nuclei from mouse brain. Species Sequence Homology: human, ferret, chick and salamander
Species Sequence Homology
Human, ferret, chick and salamander
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 12 hours after treatment. The blue staining is DAPI. MBS601235 detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.)
Testing Data
(Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 12 hours after treatment. The blue staining is DAPI. MBS601235 detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.)
Testing Data
(Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 72 hours after treatment. The blue staining is DAPI. MBS601235 was detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.)
Testing Data
(Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 72 hours after treatment. The blue staining is DAPI. MBS601235 was detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.)
Immunohistochemistry (IHC)
(MBS601235 staining pattern/morphology in rat cerebellum. Tissue pretreated with citrate, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select. Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Low magnification)
Immunohistochemistry (IHC)
(MBS601235 staining pattern/morphology in rat cerebellum. Tissue pretreated with citrate, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select. Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Low magnification)
Testing Data
Testing Data
Related Product Information for anti-Neuron Specific Nuclear Protein antibody
Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
Product Categories/Family for anti-Neuron Specific Nuclear Protein antibody
Similar Products
Product Notes
The Neuron Specific Nuclear Protein (Catalog #AAA601235) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neuron Specific Nuclear Protein (NeuN) (Biotin) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuron Specific Nuclear Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Dilution: Immunohistochemistry: 1:100; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde- based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100. Researchers should empirically determine the suitability of the Neuron Specific Nuclear Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuron Specific Nuclear Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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