Rabbit anti-Human STAT4 Polyclonal Antibody | anti-STAT4 antibody

Anti-STAT4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100961

• Gene Names: STAT4; SLEB11
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: STAT4; Polyclonal Antibody; Anti-STAT4 Antibody; Rabbit Polyclonal; STAT4 Antibody; Rabbit PAb; Antigen Affinity Purified; signal transducer and activator of transcription 4; Anti-SLEB11 Antibody; anti-STAT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human STAT4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-STAT4 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human STAT4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human STAT4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of STAT4 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human STAT4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus..)

Western Blot (WB)

(Anti-STAT4 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:86 kDaObserved band size:86 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-STAT4 antibody

High expression of STAT4 in gastric cancer predicted a better clinical outcome. STAT4 might be a useful biomarker to identify patients at high risk of recurrence after gastrectomy. Genetic variants in the transcription factor STAT4 are associated with increased susceptibility to systemic lupus erythematosus (SLE) and a more severe disease phenotype.

Product Categories/Family for anti-STAT4 antibody

Apoptosis Transcription Factors and Regulators; Jak-Stat Signaling Pathway

References

Thierfelder WE, et al. (1996) Requirement for Stat4 in interleukin-12-mediated responses of natural killer and T cells. Nature. 382(6587):171-4.Hye-Soon L, et al. (2007) Association of STAT4 with Rheumatoid Arthritis in the Korean Population. Mol Med. 13(9-10): 455-60.Chitnis T, et al. (2001) Effect of targeted disruption of STAT4 and STAT6 on the induction of experimental autoimmune encephalomyelitis. J Clin Invest. 108(5): 739-47.

NCBI and Uniprot Product Information

• NCBI GI #: 345110660
• NCBI GeneID: 6775
• NCBI Accession #: NP_001230764.1
• NCBI GenBank Nucleotide #: NM_001243835.1
• UniProt Accession #: Q14765; Q96NZ6
• Molecular Weight: 85,941 Da
• NCBI Official Full Name: signal transducer and activator of transcription 4
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4
• UniProt Protein Name: Signal transducer and activator of transcription 4
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT4
• UniProt Entry Name: STAT4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32.2-q32.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: signal transducer activity; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein amino acid phosphorylation

Disease: Systemic Lupus Erythematosus, Susceptibility To, 11