Rabbit STAMBP Polyclonal Antibody | anti-STAMBP antibody

STAMBP (STAM binding protein, AMSH, Associated molecule with the SH3 domain of STAM, Endosome-associated ubiquitin isopeptidase, MGC126516, MGC126518)

Cat. Num. AAA621480

• Gene Names: STAMBP; AMSH; MGC126516; MGC126518
• Reactivity: Human, Monkey
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A and petide affinity chromatography.
• Synonyms: STAMBP; Polyclonal Antibody; STAMBP (STAM binding protein; AMSH; Associated molecule with the SH3 domain of STAM; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518); Anti -STAMBP (STAM binding protein; anti-STAMBP antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total STAMBP protein. Species crossreactivity: Human and monkey
• Purity/Purification: Affinity Purified; Purified by Protein A and petide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-STAMBP antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Gly194 of human STAMBP protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using MBS621480.)

Related Product Information for anti-STAMBP antibody

Protein ubiquitination and deubiquitination is a reversible process catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (1,2). Deubiquitinating enzymes (DUBs) function as ubiquitin-specific proteases and can be divided into five subfamilies based on catalytic domain structure. At least 14 members of the JAMM ubiquitin protease subfamily have been identified, including signal transducing adaptor molecule (STAM) binding protein (3). STAM-binding protein (STAMBP or AMSH) is an endosomal DUB that preferentially displays ubiquitin isopeptidase activity toward K63-linked chains, which is critically dependent upon its interaction with STAM (4,5). STAMBP interacts with the STAM adaptor protein and becomes integrated into the multivesicular body sorting machinery to help regulate endosomal trafficking and receptor tyrosine kinase stability by deubiquitining target proteins (4,6). Evidence indicates that endosomal STAMBP antagonizes the ubiquitin-dependent trafficking of EGFR to the lysosomal compartment (7).

Product Categories/Family for anti-STAMBP antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 42519912
• NCBI GeneID: 10617
• NCBI Accession #: NP_964010.1
• NCBI GenBank Nucleotide #: NM_201647.2
• UniProt Accession #: O95630; Q3MJE7; D6W5H7
• Molecular Weight: 48,077 Da
• NCBI Official Full Name: STAM-binding protein
• NCBI Official Synonym Full Names: STAM binding protein
• NCBI Official Symbol: STAMBP
• NCBI Official Synonym Symbols: AMSH; MGC126516; MGC126518
• NCBI Protein Information: STAM-binding protein; OTTHUMP00000160249; OTTHUMP00000202512; OTTHUMP00000202513; endosome-associated ubiquitin isopeptidase; associated molecule with the SH3 domain of STAM
• UniProt Protein Name: STAM-binding protein
• Protein Family: STAM-binding protein
• UniProt Gene Name: STAMBP
• UniProt Synonym Gene Names: AMSH
• UniProt Entry Name: STABP_HUMAN

NCBI Description

Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Uniprot Description

AMSH: an adaptor protein involved in cytokine signal transduction the JAK-STAT cascade. Binds to the SH3 domain of the signal-transducing adaptor molecule STAM (signal transducing adaptor molecule). May play a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression.

Protein type: Protease; Ubiquitin-specific protease; EC 3.4.19.-; EC 3.1.2.15

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: nucleoplasm; early endosome; cytoplasm; plasma membrane; nucleus; cleavage furrow

Molecular Function: protein domain specific binding; protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity

Biological Process: protein deubiquitination; cytokinesis after mitosis; positive regulation of cell proliferation; negative regulation of Ras protein signal transduction; negative regulation of neuron apoptosis; proteolysis; JAK-STAT cascade; negative regulation of phosphoinositide 3-kinase cascade

Disease: Microcephaly-capillary Malformation Syndrome