Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ST8SIA4 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit anti-Human, Rat ST8SIA4 Polyclonal Antibody | anti-ST8SIA4 antibody
ST8SIA4
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ST8SIA4 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ST8SIA4 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ST8SIA4: Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells. Belongs to the glycosyltransferase 29 family.
Protein type: Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; EC 2.4.99.-; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q21
Cellular Component: Golgi membrane; integral to Golgi membrane
Molecular Function: alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity; sialic acid binding
Biological Process: axon guidance; nervous system development; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein modification process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification; oligosaccharide metabolic process; ganglioside biosynthetic process
Research Articles on ST8SIA4
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Product Notes
The ST8SIA4 st8sia4 (Catalog #AAA9125683) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ST8SIA4 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ST8SIA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ST8SIA4 st8sia4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST8SIA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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