Western Blot (WB)
(Western blot analysis of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.)
Rabbit CTSK Polyclonal Antibody | anti-CTSK antibody
CTSK Antibody (Center E112)
Western Blot (WB)
(Western blot analysis of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.)
Western Blot (WB)
(Western blot analysis of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.)
Immunohistochemistry (IHC)
(CTSK Antibody (Center E112) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTSK Antibody (Center E112) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CTSK Antibody (Center E112) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTSK Antibody (Center E112) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CTSK Antibody (Center E112) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CTSK Antibody (Center E112) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
proteinase involved in bone remodeling and resorption. This
protein, which is a member of the peptidase C1 protein family, is
predominantly expressed in osteoclasts. However, the encoded
protein is also expressed in a significant fraction of human breast
cancers, where it could contribute to tumor invasiveness. Mutations
in this gene are the cause of pycnodysostosis, an autosomal
recessive disease characterized by osteosclerosis and short
stature. This gene may be subject to RNA editing. [provided by
RefSeq].
Novinec, M., et al. Biochem. J. 429(2):379-389(2010)
Khan, B., et al. J. Investig. Med. 58(5):720-724(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Szumilo, J., et al. Folia Histochem. Cytobiol. 47(4):571-578(2009)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]
Uniprot Description
CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.
Protein type: EC 3.4.22.38; Protease
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: extracellular space; extracellular region
Molecular Function: collagen binding; proteoglycan binding; protein binding; cysteine-type endopeptidase activity; fibronectin binding; cysteine-type peptidase activity
Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; intramembranous ossification; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption
Disease: Pycnodysostosis
Research Articles on CTSK
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Product Notes
The CTSK ctsk (Catalog #AAA9207161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 97-126. The CTSK Antibody (Center E112) reacts with Human (Predicted Reactivity: Bovine, Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's CTSK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the CTSK ctsk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTSK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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