Goat BAIAP2 / IRSP53 Polyclonal Antibody | anti-BAIAP2 antibody

Goat Polyclonal to Human BAIAP2 / IRSP53

Cat. Num. AAA243875

• Gene Names: BAIAP2; BAP2; FLAF3; IRSP53
• Reactivity: Human; Predicted Reactivity: Gibbon, Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: BAIAP2 / IRSP53; Polyclonal Antibody; Goat Polyclonal to Human BAIAP2 / IRSP53; Anti-BAIAP2 / IRSP53 Antibody (Isoform 3) IHC-plus; BAIAP2; BAI-associated protein 2; BAI1-associated protein 2; BAP2; FLAF3; Fas ligand-associated factor 3; IRSP53; IRS-58; Protein BAP2; Insulin receptor substrate p53; IRSp53/58; Human BAIAP2; anti-BAIAP2 antibody

• Host: Goat
• Reactivity: Human; Predicted Reactivity: Gibbon, Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human BAIAP2 / IRSP53. This antibody will recognise only one of three reported isoforms (NP_006331.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 521

• Applicable Applications for anti-BAIAP2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 55 kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 57.4 kDa according to NP_006331.1). Recommended concentration: 1-3 ug/ml. An...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-SMSSADVEVARF from the C-terminus of human BAIAP2 / IRSP53 (NP_006331.1). Percent identity with other species by BLAST analysis: Human (100%), Gibbon (92%), Mouse (92%), Rat (92%), Hamster (92%), Bovine (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: BAIAP2 / IRSP53 antibody was raised against synthetic peptide C-SMSSADVEVARF from the C-terminus of human BAIAP2 / IRSP53 (NP_006331.1). Percent identity with other species by BLAST analysis: Human (100%), Gibbon (92%), Mouse (92%), Rat (92%), Hamster (92%), Bovine (83%).
• Antigen Modification: Isoform 3
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 9257197
• NCBI GeneID: 10458
• NCBI Accession #: NP_059344.1
• NCBI GenBank Nucleotide #: NM_017450.2
• UniProt Accession #: Q9UQB8; O43858; Q53HB1; Q86WC1; Q8N5C0; Q96CR7; Q9UBR3; Q9UQ43
• Molecular Weight: 57,430 Da
• NCBI Official Full Name: brain-specific angiogenesis inhibitor 1-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: BAI1-associated protein 2
• NCBI Official Symbol: BAIAP2
• NCBI Official Synonym Symbols: BAP2; FLAF3; IRSP53
• NCBI Protein Information: brain-specific angiogenesis inhibitor 1-associated protein 2; IRS-58; IRSp53/58; fas ligand-associated factor 3; insulin receptor substrate p53/p58; insulin receptor substrate protein of 53 kDa
• UniProt Protein Name: Brain-specific angiogenesis inhibitor 1-associated protein 2
• Protein Family: Brain-specific angiogenesis inhibitor 1-associated protein
• UniProt Gene Name: BAIAP2
• UniProt Synonym Gene Names: BAI-associated protein 2; BAI1-associated protein 2; Protein BAP2; FLAF3; IRS-58; IRSp53/58; IRSp53; Insulin receptor substrate p53
• UniProt Entry Name: BAIP2_HUMAN

NCBI Description

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

IRSp53: Adapter protein that links membrane-bound small G- proteins to cytoplasmic effector proteins. Necessary for CDC42- mediated reorganization of the actin cytoskeleton and for RAC1- mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1, BAI1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Interacts with E.coli intimin receptor Tir. Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; ruffle; neuron projection; cytoplasm; plasma membrane; cytosol; filopodium; actin cytoskeleton

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; cytoskeletal adaptor activity

Biological Process: actin crosslink formation; actin filament bundle formation; regulation of cell shape; plasma membrane organization and biogenesis; response to bacterium; axonogenesis; regulation of actin cytoskeleton organization and biogenesis; dendrite development; insulin receptor signaling pathway; innate immune response; regulation of synaptic plasticity; vascular endothelial growth factor receptor signaling pathway