Western Blot (WB)
(Western Blot analysis of extracts of various cells, using SSX5 antibody.)
Rabbit SSX5 Polyclonal Antibody | anti-SSX5 antibody
SSX5 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SSX5; Polyclonal Antibody; SSX5 Polyclonal Antibody; Protein SSX5; anti-SSX5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SSX5 polyclonal antibody detects endogenous levels of SSX5 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
229
Applicable Applications for anti-SSX5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
IF: 1:50-1:100
Immunogen
Recombinant protein of human SSX5.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using SSX5 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using SSX5 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using SSX5 antibody at dilution of 1:100 (x400 lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using SSX5 antibody at dilution of 1:100 (x400 lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cell using SSX5 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cell using SSX5 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-SSX5 antibody
The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. Two transcript variants encoding distinct isoforms have been identified for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 22kDa
NCBI Official Full Name
protein SSX5 isoform a
UniProt Protein Name
Protein SSX5
Protein Family
UniProt Gene Name
SSX5
Uniprot Description
Ssxb8: Could act as a modulator of transcription. Belongs to the SSX family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: nucleus
Molecular Function: nucleic acid binding; protein binding; transcription corepressor activity
Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent
Similar Products
Product Notes
The SSX5 ssx5 (Catalog #AAA3008841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SSX5 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SSX5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:100. Researchers should empirically determine the suitability of the SSX5 ssx5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSX5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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