Western Blot (WB)
(Biotinylated (0.2ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat SSP29/ANP32B Polyclonal Antibody | anti-ANP32B antibody
Goat anti-SSP29/ANP32B, Biotinylated Antibody
Gene Names
ANP32B; APRIL; SSP29; PHAPI2
Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SSP29/ANP32B; Polyclonal Antibody; Goat anti-SSP29/ANP32B; Biotinylated Antibody; Goat Anti-SSP29 / ANP32B Antibody; ANP32B; acidic nuclear phosphoprotein 32 family member B; APRIL; PHAPI2; SSP29; acidic (leucine-rich) nuclear phosphoprotein 32 family; member B; acidic protein rich in leucines; putative HLA-DR-associated protein I-2; silver-stainable protein SSP29; ANP32B antibody; acidic nuclear phosphoprotein 32 family member B antibody; APRIL antibody; PHAPI2 antibody; SSP29 antibody; member B antibody; acidic protein rich in leucines antibody; putative HLA-DR-associated protein I-2 antibody; silver-stainable protein SSP29 antibody; SSP29 / ANP32B; Biotinylated; anti-ANP32B antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KRKRETDDEGEDD
Sequence Length
251
Applicable Applications for anti-ANP32B antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 28kDa band observed in Human Tonsil lysates (calculated MW of 28.8kDa according to NP_006392.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 28kDa band observed in Human Tonsil lysates (calculated MW of 28.8kDa according to NP_006392.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-KRKRETDDEGEDD. , from the C Terminus of the protein sequence according to NP_006392.1.
Epitope
C Terminus
Note
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Biotinylated (0.2ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.2ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,277 Da
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member B
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member B
NCBI Official Symbol
ANP32B
NCBI Official Synonym Symbols
APRIL; SSP29; PHAPI2
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member B
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member B
Protein Family
UniProt Gene Name
ANP32B
UniProt Synonym Gene Names
APRIL; PHAPI2; PHAPI2
UniProt Entry Name
AN32B_HUMAN
Uniprot Description
ANP32B: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Belongs to the ANP32 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Chaperone
Chromosomal Location of Human Ortholog: 9q22.32
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: histone binding; protein binding
Biological Process: caspase activation; inner ear development; negative regulation of cell differentiation; nucleosome assembly; palate development; positive regulation of protein export from nucleus; vasculature development; ventricular system development
Research Articles on ANP32B
Similar Products
Product Notes
The ANP32B anp32b (Catalog #AAA423521) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SSP29/ANP32B, Biotinylated Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SSP29/ANP32B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 28kDa band observed in Human Tonsil lysates (calculated MW of 28.8kDa according to NP_006392.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ANP32B anp32b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRKRETDDEG EDD. It is sometimes possible for the material contained within the vial of "SSP29/ANP32B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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