Rabbit anti-Human SSH3 Polyclonal Antibody | anti-SSH3 antibody

SSH3, CT (SSH3, SSH3L, Protein phosphatase Slingshot homolog 3, SSH-like protein 3) (PE)

Cat. Num. AAA6351344

• Gene Names: SSH3; SSH3L
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SSH3; Polyclonal Antibody; CT (SSH3; SSH3L; Protein phosphatase Slingshot homolog 3; SSH-like protein 3) (PE); SSH-like protein 3; anti-SSH3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SSH3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SSH3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).)

Immunohistochemistry (IHC)

(SSH3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SSH3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-SSH3 antibody

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).

Product Categories/Family for anti-SSH3 antibody

Antibodies; Actin Related Proteins

References

Kousaka, K., et al. Genesis 46(5):246-255(2008). Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Soosairajah, J., et al. EMBO J. 24(3):473-486(2005). Ohta, Y., et al. Genes Cells 8(10):811-824(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 239582767
• NCBI GeneID: 54961
• NCBI Accession #: NP_060327.3
• NCBI GenBank Nucleotide #: NM_017857.3
• UniProt Accession #: Q8TE77; Q6PK42; Q76I75; Q8N9L8; Q8WYL0; Q9NV45; Q9NWZ7
• Molecular Weight: 72,996 Da
• NCBI Official Full Name: protein phosphatase Slingshot homolog 3
• NCBI Official Synonym Full Names: slingshot protein phosphatase 3
• NCBI Official Symbol: SSH3
• NCBI Official Synonym Symbols: SSH3L
• NCBI Protein Information: protein phosphatase Slingshot homolog 3; SSH-3L; hSSH-3L; slingshot 3; SSH-like protein 3; slingshot homolog 3
• UniProt Protein Name: Protein phosphatase Slingshot homolog 3
• Protein Family: Protein phosphatase Slingshot
• UniProt Gene Name: SSH3
• UniProt Synonym Gene Names: SSH3L; SSH-3L; hSSH-3L
• UniProt Entry Name: SSH3_HUMAN

NCBI Description

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM, Mar 2008]

Uniprot Description

SSH3: a protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Does not bind to, or colocalize with, filamentous actin. Five alternatively spliced isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; Protein phosphatase, Ser/Thr (non-receptor); Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.48; Cytoskeletal; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytoskeleton; cytoplasm; nucleus

Molecular Function: DNA binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; actin binding

Biological Process: regulation of actin polymerization and/or depolymerization; regulation of axonogenesis; protein amino acid dephosphorylation