Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot: Sample: Recombinant MPO, Rat.)

Rabbit Myeloperoxidase Polyclonal Antibody | anti-MPO antibody

Polyclonal Antibody to Myeloperoxidase (MPO)

Reactivity
Rattus norvegicus (Rat)
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Myeloperoxidase; Polyclonal Antibody; Polyclonal Antibody to Myeloperoxidase (MPO); anti-MPO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rattus norvegicus (Rat)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MPO antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human; Mouse
Immunogen
Recombinant MPO (Val134~Gly263 (Accession # D3ZGE2)) expressed in E.coli (MBS2030649).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C.

Stability Test:
Thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant MPO, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant MPO, Rat.)

Western Blot (WB)

(Western Blot: Sample: Human Hela Cells.)

Western Blot (WB) (Western Blot: Sample: Human Hela Cells.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Spleen Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Spleen Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myeloperoxidase
NCBI Official Synonym Full Names
myeloperoxidase
NCBI Official Symbol
Mpo
NCBI Protein Information
myeloperoxidase
UniProt Protein Name
Myeloperoxidase
Protein Family
UniProt Gene Name
Mpo

NCBI Description

myeloperoxidase family member that may contribute to hyperoxia-mediated lung injury via nitration of proteins, resulting in reactive nitrogen species [RGD, Feb 2006]

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: azurophil granule; cytoplasm; extracellular space; intracellular membrane-bound organelle; mitochondrion; nucleus; secretory granule

Molecular Function: heme binding; heparin binding; peroxidase activity

Biological Process: aging; defense response; defense response to bacterium; defense response to fungus; defense response to Gram-positive bacterium; hydrogen peroxide catabolic process; removal of superoxide radicals; respiratory burst involved in defense response; response to food; response to lipopolysaccharide; response to mechanical stimulus; response to oxidative stress; response to yeast

Research Articles on MPO

Similar Products

Product Notes

The MPO mpo (Catalog #AAA2028623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Myeloperoxidase (MPO) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Myeloperoxidase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MPO mpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myeloperoxidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.