Rabbit SSA1/Ro-52 Polyclonal Antibody | anti-TRIM21 antibody

SSA1/Ro-52 (Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

Cat. Num. AAA611465

• Gene Names: TRIM21; SSA; RO52; SSA1; RNF81
• Reactivity: Human, Rodent
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: SSA1/Ro-52; Polyclonal Antibody; SSA1/Ro-52 (Sjogren Syndrome Antigen A1; 52kD Ribonucleoprotein Autoantigen SS-A/Ro; RO52; RNF81; Tripartite Motif Protein 21; TRIM21); Anti -SSA1/Ro-52 (Sjogren Syndrome Antigen A1; anti-TRIM21 antibody

• Host: Rabbit
• Reactivity: Human, Rodent
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SSA1. Species Crossreactivity: rodent.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, sodium azide, 40% glycerol.

• Applicable Applications for anti-TRIM21 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA (serum titer): 1:125,000.
• Immunogen: Synthetic peptide, WNKQKYEAGTYPQTPL of human SSA1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-TRIM21 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 14994115
• NCBI GeneID: 6737
• UniProt Accession #: P19474; Q5XPV5; Q96RF8
• Molecular Weight: 54,170 Da
• NCBI Official Full Name: SSA1
• NCBI Official Synonym Full Names: tripartite motif containing 21
• NCBI Official Symbol: TRIM21
• NCBI Official Synonym Symbols: SSA; RO52; SSA1; RNF81
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; OTTHUMP00000230775; RING finger protein 81; Sicca syndrome antigen A; tripartite motif-containing 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM21
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM21
• UniProt Synonym Gene Names: RNF81; RO52; SSA1
• UniProt Entry Name: RO52_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Uniprot Description

TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; cytoplasm; SCF ubiquitin ligase complex; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein monoubiquitination; regulation of interferon type I production; protein autoubiquitination; protein polyubiquitination; protein destabilization; cytokine and chemokine mediated signaling pathway; positive regulation of cell cycle; protein ubiquitination; cell cycle; negative regulation of viral transcription; positive regulation of virion penetration into host cell; inhibition of NF-kappaB transcription factor; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity