Rabbit anti-Human SRY Polyclonal Antibody | anti-SRY antibody

SRY Antibody (Center)

Cat. Num. AAA9216319

• Gene Names: SRY; TDF; TDY; SRXX1; SRXY1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: SRY; Polyclonal Antibody; SRY Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Sex-determining region Y protein; Testis-determining factor; TDF; anti-SRY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This SRY antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-112 amino acids from the Central region of human SRY.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 204

• Applicable Applications for anti-SRY antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:1000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-SRY Antibody (Center)at 1:1000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-SRY antibody

Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity). Involved in different aspects of gene regulation including promoter activation or repression (By similarity). Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.

Product Categories/Family for anti-SRY antibody

Developmental Biology

References

Sinclair A.H.,et al.Nature 346:240-244(1990). Su H.,et al.Am. J. Hum. Genet. 52:24-38(1993). Behlke M.A.,et al.Genomics 17:736-739(1993). Whitfield L.S.,et al.Genomics 27:306-311(1995). Ferrari S.,et al.EMBO J. 11:4497-4506(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4507225
• NCBI GeneID: 6736
• NCBI Accession #: NP_003131.1
• NCBI GenBank Nucleotide #: NM_003140.2
• UniProt Accession #: Q05066
• Molecular Weight: 23884
• NCBI Official Full Name: sex-determining region Y protein
• NCBI Official Synonym Full Names: sex determining region Y
• NCBI Official Symbol: SRY
• NCBI Official Synonym Symbols: TDF; TDY; SRXX1; SRXY1
• NCBI Protein Information: sex-determining region Y protein
• UniProt Protein Name: Sex-determining region Y protein
• Protein Family: SRY-related protein
• UniProt Gene Name: SRY
• UniProt Synonym Gene Names: TDF
• UniProt Entry Name: SRY_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

SRY: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. Defects in SRY are the cause of 46,XY sex reversal type 1 (SRXY1). A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Patients manifest rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, streak gonads, variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female. Defects in SRY are the cause of 46,XX sex reversal type 1 (SRXX1). A condition in which male gonads develop in a genetic female (female to male sex reversal). Belongs to the SRY family.

Protein type: Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: Yp11.3

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; calmodulin binding; DNA binding; protein heterodimerization activity; DNA bending activity; transcription factor binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; male sex determination; male gonad development; negative regulation of transcription from RNA polymerase II promoter; cell differentiation

Disease: 46,xx Sex Reversal 1; 46,xy Sex Reversal 1