Rabbit SRI / Sorcin Polyclonal Antibody | anti-SRI antibody

Rabbit Polyclonal to Human SRI / Sorcin

Cat. Num. AAA241684

• Gene Names: SRI; SCN; V19; CP22; CP-22
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Opossum, Chicken, Xenopus
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SRI / Sorcin; Polyclonal Antibody; Rabbit Polyclonal to Human SRI / Sorcin; Anti-SRI / Sorcin Antibody (aa50-100) IHC-plus; SRI; 22 kDa protein; CP22; CP-22; H_RG167B05.1; SCN; V19; Sorcin; Human SRI; anti-SRI antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Opossum, Chicken, Xenopus
• Clonality: Polyclonal
• Specificity: A portion of amino acids 50-100 of human Sorcin
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.2% gelatin, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 198

• Applicable Applications for anti-SRI antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (0.5 - 2 ug/ml); ; Optimal dilution to be determined by the researcher. ; ; Usage Summary: Immunohistochemistry: MBS241684 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241684 was determined to be 10ug/ml.
• Antigen Type: Synthetic peptide
• Immunogen: SRI / Sorcin antibody was raised against a portion of amino acids 50-100 of human Sorcin.
• Epitope: aa50-100
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SRI antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of human Sorcin in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507207
• NCBI GeneID: 6717
• NCBI Accession #: NP_003121.1
• NCBI GenBank Nucleotide #: NM_003130.3
• UniProt Accession #: P30626; A8MTH6; B4DKK2; D6W5Q0
• NCBI Official Full Name: sorcin isoform A
• NCBI Official Synonym Full Names: sorcin
• NCBI Official Symbol: SRI
• NCBI Official Synonym Symbols: SCN; V19; CP22; CP-22
• NCBI Protein Information: sorcin; H_RG167B05.1; 22 kDa protein; calcium binding protein amplified in mutlidrug-resistant cells
• UniProt Protein Name: Sorcin
• UniProt Gene Name: SRI
• UniProt Synonym Gene Names: CP22
• UniProt Entry Name: SORCN_HUMAN

NCBI Description

This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

SRI: Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; membrane; mitochondrion; sarcoplasmic reticulum; T-tubule; cytoplasm; nerve terminal; cytosol; Z disc

Molecular Function: protein binding; protease binding; protein heterodimerization activity; calcium channel regulator activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: regulation of action potential; cytoplasmic sequestering of transcription factor; muscle development; negative regulation of heart rate; heart development; regulation of striated muscle contraction; regulation of calcium ion transport; signal transduction; proteolysis; regulation of heart contraction; transport; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol; intracellular sequestering of iron ion