Rabbit anti-Human SREBF2 Polyclonal Antibody | anti-SREBF2 antibody

SREBF2, ID (SREBF2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2) (Azide free) (HRP)

Cat. Num. AAA6351140

• Gene Names: SREBF2; SREBP2; bHLHd2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SREBF2; Polyclonal Antibody; ID (SREBF2; BHLHD2; SREBP2; Sterol regulatory element-binding protein 2; Class D basic helix-loop-helix protein 2; Sterol regulatory element-binding transcription factor 2; Processed sterol regulatory element-binding protein 2) (Azide free) (HRP); Processed sterol regulatory element-binding protein 2; anti-SREBF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SREBF2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SREBF2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 399-427 in the central region of human SREBF2, UniProt Accession # NP_004590.2; Q12772.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS648042. This demonstrates that MBS648042 detected the SREBF2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma using MBS648042 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648042 for immunohistochemistry.)

Related Product Information for anti-SREBF2 antibody

SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.

Product Categories/Family for anti-SREBF2 antibody

Antibodies; Transcription Factors

References

1. Gerin, I., et al. J. Biol. Chem. 285(44):33652-33661(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Marquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12228-12232(2010). 4. Najafi-Shoushtari, S.H., et al. Science 328(5985):1566-1569(2010). 5. Pramfalk, C., et al. J. Lipid Res. 51(6):1354-1362(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 27477113
• NCBI GeneID: 6721
• NCBI Accession #: NP_004590.2
• NCBI GenBank Nucleotide #: NM_004599.2
• UniProt Accession #: Q12772; Q6GTH7; Q86V36; Q9UH04; Q05BD5; Q8NCY3
• Molecular Weight: 123,688 Da
• NCBI Official Full Name: sterol regulatory element-binding protein 2
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 2
• NCBI Official Symbol: SREBF2
• NCBI Official Synonym Symbols: SREBP2; bHLHd2
• NCBI Protein Information: sterol regulatory element-binding protein 2; SREBP-2; OTTHUMP00000199514; class D basic helix-loop-helix protein 2; sterol regulatory element-binding transcription factor 2
• UniProt Protein Name: Sterol regulatory element-binding protein 2
• Protein Family: Sterol regulatory element-binding protein
• UniProt Gene Name: SREBF2
• UniProt Synonym Gene Names: BHLHD2; SREBP2; SREBP-2; bHLHd2
• UniProt Entry Name: SRBP2_HUMAN

Uniprot Description

SREBP-2: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. Belongs to the SREBP family.

Protein type: Transcription factor; Membrane protein, multi-pass; Membrane protein, integral; DNA-binding

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; C-8 sterol isomerase activity

Biological Process: cholesterol metabolic process; transcription, DNA-dependent; response to low density lipoprotein stimulus; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; lipid metabolic process; cellular lipid metabolic process