Rabbit anti-Human SREBF1 Polyclonal Antibody | anti-SREBF1 antibody

SREBF1, ID (Processed Sterol Regulatory Element-binding Protein 1, Class D Basic Helix-loop-helix Protein 1, bHLHd1, Sterol Regulatory Element-binding Transcription Factor 1, Sterol Regulatory Element-binding Protein 1, SREBP-1, BHLHD1) (Biotin)

Cat. Num. AAA6500702

• Gene Names: SREBF1; SREBP1; bHLHd1; SREBP-1c
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SREBF1; Polyclonal Antibody; ID (Processed Sterol Regulatory Element-binding Protein 1; Class D Basic Helix-loop-helix Protein 1; bHLHd1; Sterol Regulatory Element-binding Transcription Factor 1; Sterol Regulatory Element-binding Protein 1; SREBP-1; BHLHD1) (Biotin); anti-SREBF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SREBF1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SREBF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SREBF1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SREBF1 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). SREBF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SREBF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SREBF1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SREBF1 antibody

Antibodies; Transcription Factors

References

1. Wang, X., et.al., Cell 77(1), 53-62 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 256665251
• NCBI GeneID: 6720
• NCBI Accession #: NP_004167.3
• NCBI GenBank Nucleotide #: NM_004176
• UniProt Accession #: P36956; Q16062; Q59F52; Q6P4R7; Q6PFW7; Q6PJ36; Q8TAK9; D3DXC4
• Molecular Weight: 121,675 Da
• NCBI Official Full Name: Homo sapiens sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 1
• NCBI Official Symbol: SREBF1
• NCBI Official Synonym Symbols: SREBP1; bHLHd1; SREBP-1c
• NCBI Protein Information: sterol regulatory element-binding protein 1; SREBP-1; class D basic helix-loop-helix protein 1
• UniProt Protein Name: Sterol regulatory element-binding protein 1
• UniProt Gene Name: SREBF1
• UniProt Synonym Gene Names: BHLHD1; SREBP1; SREBP-1; bHLHd1
• UniProt Entry Name: SRBP1_HUMAN

Uniprot Description

SREBP-1: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. Belongs to the SREBP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cytoplasm; integral to membrane; nuclear envelope; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; sterol response element binding; DNA binding; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: response to food; response to drug; circadian rhythm; cholesterol metabolic process; response to glucagon stimulus; response to retinoic acid; response to cAMP; transcription, DNA-dependent; lipid biosynthetic process; positive regulation of cholesterol biosynthetic process; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; cellular response to starvation; negative regulation of insulin secretion; regulation of transcription from RNA polymerase II promoter; positive regulation of histone deacetylation; chemical signal regulation of heart rate; regulation of fatty acid metabolic process; response to glucose stimulus; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; response to progesterone stimulus; lung development; aging