Rabbit anti-Human, Mouse SRD5A2 Polyclonal Antibody | anti-SRD5A2 antibody

SRD5A2, ID (SRD5A2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, SR type 2, Steroid 5-alpha-reductase 2, Type II 5-alpha reductase) (FITC)

Cat. Num. AAA6351106

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SRD5A2; Polyclonal Antibody; ID (SRD5A2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; Type II 5-alpha reductase) (FITC); Type II 5-alpha reductase; anti-SRD5A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SRD5A2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SRD5A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from the Central region of human SRD5A2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SRD5A2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the SRD5A2 antibody detected SRD5A2 protein (arrow).)

Related Product Information for anti-SRD5A2 antibody

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Product Categories/Family for anti-SRD5A2 antibody

Antibodies; Enzymes; Dehydrogenase

References

Thigpen, A.E., et al. N. Engl. J. Med. 327(17):1216-1219(1992) Thigpen, A.E., et al. J. Clin. Invest. 90(3):799-809(1992) Labrie, F., et al. Endocrinology 131(3):1571-1573(1992) Andersson, S., et al. Nature 354(6349):159-161(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 39812447
• NCBI GeneID: 6716
• NCBI Accession #: NP_000339.2
• NCBI GenBank Nucleotide #: NM_000348.3
• UniProt Accession #: P31213; Q2M1R4; Q9BYE6; B2RE87
• Molecular Weight: 28,393 Da
• NCBI Official Full Name: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
• NCBI Official Synonym Full Names: steroid 5 alpha-reductase 2
• NCBI Official Symbol: SRD5A2
• NCBI Protein Information: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
• UniProt Protein Name: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
• Protein Family: 3-oxo-5-alpha-steroid 4-dehydrogenase
• UniProt Gene Name: SRD5A2
• UniProt Synonym Gene Names: S5AR 2

NCBI Description

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]

Uniprot Description

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.