Rabbit anti-Human SERPINH1 Polyclonal Antibody | anti-SERPINH1 antibody

SERPINH1, ID (Serpin H1, Collagen-binding Protein, Colligin, 47kD Heat Shock Protein, Rheumatoid Arthritis-related Antigen RA-A47, Arsenic-transactivated Protein 3, AsTP3, Cell Proliferation-inducing Gene 14 Protein, PIG14, SERPINH2, HSP47, CBP2, CBP1) (M

Cat. Num. AAA6499018

• Gene Names: SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SERPINH1; Polyclonal Antibody; ID (Serpin H1; Collagen-binding Protein; Colligin; 47kD Heat Shock Protein; Rheumatoid Arthritis-related Antigen RA-A47; Arsenic-transactivated Protein 3; AsTP3; Cell Proliferation-inducing Gene 14 Protein; PIG14; SERPINH2; HSP47; CBP2; CBP1) (M; anti-SERPINH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SERPINH1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-SERPINH1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SERPINH1 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SERPINH1 antibody (Center) in HepG2 cell line lysates (35ug/lane). SERPINH1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SERPINH1 Antibody (Center) (RB18952) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINH1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SERPINH1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SERPINH1 antibody

Antibodies; Heat Shock Proteins

References

1. Araki, K., Br. J. Cancer 101(3), 492-497 (2009). 2. Katagata, Y., J. Dermatol. Sci. 49(1), 33-38 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 32454741
• NCBI GeneID: 871
• NCBI Accession #: NP_001226
• NCBI GenBank Nucleotide #: NM_001235.3
• UniProt Accession #: P50454; P29043; Q5XPB4; Q6NSJ6; Q8IY96; Q9NP88; B3KVJ3
• Molecular Weight: 48.9 kDa (439aa), confirmed by MALDI-TOF
• NCBI Official Full Name: serpin H1
• NCBI Official Synonym Full Names: serpin family H member 1
• NCBI Official Symbol: SERPINH1
• NCBI Official Synonym Symbols: CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
• NCBI Protein Information: serpin H1
• UniProt Protein Name: Serpin H1
• Protein Family: Serpin
• UniProt Gene Name: SERPINH1
• UniProt Synonym Gene Names: CBP1; CBP2; HSP47; SERPINH2; AsTP3; Colligin
• UniProt Entry Name: SERPH_HUMAN

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Uniprot Description

SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment

Molecular Function: serine-type endopeptidase inhibitor activity; collagen binding; unfolded protein binding

Biological Process: extracellular matrix organization and biogenesis; collagen fibril organization; protein maturation; collagen biosynthetic process; response to unfolded protein

Disease: Osteogenesis Imperfecta, Type X; Preterm Premature Rupture Of The Membranes