Western Blot/Blotting
(Western blot analysis using SR antibody at 1 ug/ml on canine microsomal protein.)
Sheep anti-Dog SR alpha Polyclonal Antibody | anti-SR alpha antibody
SR alpha
Reactivity
Dog
Applications
Western Blot
Purity
Ammonium Sulfate Precipitation
Synonyms
SR alpha; Polyclonal Antibody; Signal Recognition Particle Receptor; subunit; anti-SR alpha antibody
Host
Sheep
Reactivity
Dog
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Ammonium Sulfate Precipitation
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
638
Applicable Applications for anti-SR alpha antibody
Western Blot (WB)
Immunogen
The antibody to SRalpha was raised against a recombinant protein corresponding to amino acids 39-295 which includes part of the amino terminal SRbeta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.
Positive Control
Canine microsomal protein
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Western Blot/Blotting
(Western blot analysis using SR antibody at 1 ug/ml on canine microsomal protein.)
Western Blot/Blotting
(Western blot analysis using SR antibody at 1 ug/ml on canine microsomal protein.)
Related Product Information for anti-SR alpha antibody
The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.
Product Categories/Family for anti-SR alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
72
NCBI Official Full Name
Signal recognition particle receptor subunit alpha
UniProt Protein Name
Signal recognition particle receptor subunit alpha
UniProt Gene Name
SRPR
UniProt Synonym Gene Names
SR-alpha; DP-alpha
UniProt Entry Name
SRPR_CANFA
Uniprot Description
Function: Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system.
Subunit structure: Heterodimer with SRPRB.
Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Thought to be anchored in the membrane through an interaction with SR-beta, which contains a bona fide transmembrane domain.
Sequence similarities: Belongs to the GTP-binding SRP family.
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Product Notes
The SR alpha srpr (Catalog #AAA395988) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The SR alpha reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's SR alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the SR alpha srpr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SR alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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