Rabbit SR-4 Polyclonal Antibody | anti-SR-4 antibody

SR-4 (D47) Polyclonal Antibody

Cat. Num. AAA3002538

• Gene Names: HTR4; 5-HT4; 5-HT4R
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SR-4; Polyclonal Antibody; SR-4 (D47) Polyclonal Antibody; 5-hydroxytryptamine receptor 4; 5-HT-4; 5-HT4; Serotonin receptor 4; HTR4; anti-SR-4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SR-4 (D47) polyclonal antibody detects endogenous levels of SR-4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 388

• Applicable Applications for anti-SR-4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 14-66 of Human SR-4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SR-4 (D47) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of SR-4 (D47) pAb in paraffin-embedded human brain tissue.)

Western Blot (WB)

(Western Blot (WB) analysis of SR-4 (D47) pAb at 1:1000 dilutionLane1:The brain tissue lysate of Mouse (30ug) Lane2:The brain tissue lysate of Rat (30ug))

Related Product Information for anti-SR-4 antibody

Serotonin (also designated 5-hydroxytryptamine or 5-HT) is a molecule that functions as a neurotransmitter, a hormone and a mitogen, and it is predominantly expressed in the gut, platelets and central nervous system (CNS). In the CNS, serotonin modulates several processes, including anxiety, sleep, appetite, behavior and drug abuse. In platelets and gut, serotonin plays a major role in cardiovascular function and motility of the gastrointestinal tract, respectively. Serotonin mediates its effects through several of G protein coupled receptors, designated 5-HT receptors or alternatively SR receptors. SR-3 is a ligand-gated ion channel, whereas all other known serotonin receptor subtypes are G protein-coupled receptors. The gene which encodes SR-3 maps to human chromosome 11q23.1-q23.2. SR-4 mediates widespread effects in central and peripheral nervous systems. The gene which encodes SR-4 maps to human chromosome 5q31-q33. SR-7 belongs to the superfamily of G protein-coupled receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 11321563
• NCBI GeneID: 3360
• NCBI Accession #: NP_000861.1
• NCBI GenBank Nucleotide #: NP_000861.1
• UniProt Accession #: Q13639
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: 5-hydroxytryptamine receptor 4 isoform b
• NCBI Official Synonym Full Names: 5-hydroxytryptamine receptor 4
• NCBI Official Symbol: HTR4
• NCBI Official Synonym Symbols: 5-HT4; 5-HT4R
• NCBI Protein Information: 5-hydroxytryptamine receptor 4
• UniProt Protein Name: 5-hydroxytryptamine receptor 4
• UniProt Gene Name: HTR4
• UniProt Synonym Gene Names: 5-HT-4; 5-HT4
• UniProt Entry Name: 5HT4R_HUMAN

NCBI Description

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]

Uniprot Description

5-HT(4): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; endosome

Molecular Function: serotonin receptor activity

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cell proliferation; regulation of appetite; serotonin receptor signaling pathway