Testing Data
(All lanes : MBS626358 at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human SQSTM1 Polyclonal Antibody | anti-SQSTM1 antibody
SQSTM1, CT (p62) Sequestosome-1, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62, EBI3-associated Protein of 60kD, p60, EBIAP, ORCA, OSIL)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:50-1:100
Testing Data
(All lanes : MBS626358 at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes : MBS626358 at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes : MBS626358 at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: C2C12 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes : MBS626358 at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: C2C12 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(MBS626358 staining SQSTM1 in Human prostate tissue sections by Immunohistochemistry (paraformaldehyde- fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS626358 (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS626358 staining SQSTM1 in Human prostate tissue sections by Immunohistochemistry (paraformaldehyde- fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS626358 (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS626358, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS626358 for immunohistochemistry; BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS626358, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS626358 for immunohistochemistry; BC = breast carcinoma; HC = hepatocarcinoma.)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with MBS626358. U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS626358 (1:200, 2h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with MBS626358. U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS626358 (1:200, 2h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
Uniprot Description
SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Autophagy; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: nucleoplasm; PML body; endoplasmic reticulum; lysosome; cytoplasm; late endosome; autophagic vacuole; cytoplasmic vesicle; inclusion body; cytosol
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; ubiquitin binding; protein homodimerization activity; protein kinase C binding; zinc ion binding; SH2 domain binding; receptor tyrosine kinase binding; protein kinase binding
Biological Process: regulation of Ras protein signal transduction; ubiquitin-dependent protein catabolic process; nerve growth factor receptor signaling pathway; macroautophagy; positive regulation of apoptosis; immune system process; protein heterooligomerization; endosome transport; protein amino acid phosphorylation; protein localization; autophagy; response to stress; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; cell differentiation; positive regulation of macroautophagy; negative regulation of apoptosis
Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone
Research Articles on SQSTM1
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Product Notes
The SQSTM1 sqstm1 (Catalog #AAA626358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SQSTM1, CT (p62) Sequestosome-1, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62, EBI3-associated Protein of 60kD, p60, EBIAP, ORCA, OSIL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SQSTM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Dilution: ELISA: 1:1000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:50-1:100. Researchers should empirically determine the suitability of the SQSTM1 sqstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SQSTM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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