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Western Blot (WB)

Mouse VSNL1 Monoclonal Antibody | anti-VSNL1 antibody

VSNL1 Antibody

Gene Names
VSNL1; HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
Reactivity
Human, cow, mouse and rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity purified
Synonyms
VSNL1; Monoclonal Antibody; VSNL1 Antibody; anti-VSNL1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, cow, mouse and rat
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
191
Applicable Applications for anti-VSNL1 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
The antibody solution can be used at dilutions of 1:500-1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000-2,000.
Immunogen
Visinin was originally isolated biochemically from chicken retina as a major protein of about 24kDa on SDS-PAGE (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2, 3). One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamly of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury (6, 7, 8). The HGNC name for this protein is VSNL1.
Storage Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

Western Blot (WB)

Testing Data #2

Testing Data #2
References
1. Hatakenaka S, Kuo CH, Miki N. Analysis of a distinctive protein in chick retina during development. Brain Res. 312:155-63 (1983).2. Kuno T, Kajimoto Y, Hashimoto T, Mukai H, Shirai Y, Saheki S, Tanaka C. cDNA cloning of a neural visinin-like Ca(2+)-binding protein. Biochem Biophys Res Commun. 184:1219-25 (1992).3. Polymeropoulos MH, Ide S, Soares MB, Lennon GG. Sequence characterization and genetic mapping of the human VSNL1 gene, a homologue of the rat visinin-like peptide RNVP1. Genomics. 29:273-5 (1995).4. Bernstein HG, Baumann B, Danos P, Diekmann S, Bogerts B, Gundelfinger ED, Braunewell KH. Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain. J Neurocytol. 28:655-62 (1999).5. Paterlini M, Revilla V, Grant AL, Wisden W Expression of the neuronal calcium sensor protein family in the rat brain..Neuroscience. 99:205-16 (2000).6. Laterza OF, Modur VR, Crimmins DL, Olander JV, Landt Y, Lee JM, Ladenson JH. Identification of novel brain biomarkers. Clin. Chem. 9:1713-21 (2006).7. Lee JM, Blennow K, Andreasen N, Laterza O, Modur V, Olander J, Gao F, Ohlendorf M, Ladenson JH. The brain injury biomarker VLP-1 is increased in the cerebrospinal fluid of Alzheimer disease patients. Clin. Chem. 10:1617-23 (2008).8. Tarawneh R, D'Angelo G, Macy E, Xiong C, Carter D, Cairns NJ, Fagan AM, Head D, Mintun MA, Ladenson JH, Lee JM, Morris JC, Holtzman DM. Visinin-like protein-1: diagnostic and prognostic biomarker in Alzheimer disease. Ann Neurol. 70:274-85 (2011) doi: 10.1002/ana.22448.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,142 Da
NCBI Official Full Name
visinin-like protein 1
NCBI Official Synonym Full Names
visinin-like 1
NCBI Official Symbol
VSNL1
NCBI Official Synonym Symbols
HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
NCBI Protein Information
visinin-like protein 1; VLP-1; hippocalcin-like protein 3
UniProt Protein Name
Visinin-like protein 1
Protein Family
UniProt Gene Name
VSNL1
UniProt Synonym Gene Names
VISL1; VILIP; VLP-1; HLP3
UniProt Entry Name
VISL1_HUMAN

NCBI Description

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner

By similarity.

Tissue specificity: Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level). Ref.6

Miscellaneous: Probably binds three calcium ions.

Sequence similarities: Belongs to the recoverin family.Contains 4 EF-hand domains.

Research Articles on VSNL1

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Product Notes

The VSNL1 vsnl1 (Catalog #AAA415170) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VSNL1 Antibody reacts with Human, cow, mouse and rat and may cross-react with other species as described in the data sheet. AAA Biotech's VSNL1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). The antibody solution can be used at dilutions of 1:500-1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000-2,000. Researchers should empirically determine the suitability of the VSNL1 vsnl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VSNL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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