Rabbit Sprouty 4 Polyclonal Antibody | anti-SPRY4 antibody

Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4)

Cat. Num. AAA610307

• Gene Names: SPRY4; SPRY4
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Sprouty 4; Polyclonal Antibody; Sprouty 4 (Sprouty-4; Sprouty Homolog 4; Spry 4; SPRY4; SPRY-4); Anti -Sprouty 4 (Sprouty-4; anti-SPRY4 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes Human Sprouty (Drosophila) Homolog 4 (SPRY4). Species Crossreactivity: Bovine, Dog, Human, Mouse, Rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

• Applicable Applications for anti-SPRY4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry: 2.5ug/ml; Western Blot: 1:100-1:1000; ELISA: 1:3000-1:12,000
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: Amino acids 306-322 of Human Sprouty-4 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(SPRY4 (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human heart, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-SPRY4 antibody

Antibodies; Abs to Sprouty

NCBI and Uniprot Product Information

• NCBI GI #: 42795685
• NCBI GeneID: 81848
• UniProt Accession #: Q9BXY1; Q9C004
• Molecular Weight: 8,771 Da
• NCBI Official Full Name: sprouty 4
• NCBI Official Synonym Full Names: sprouty homolog 4 (Drosophila)
• NCBI Official Symbol: SPRY4
• NCBI Official Synonym Symbols: SPRY4
• NCBI Protein Information: protein sprouty homolog 4; spry-4; OTTHUMP00000160069; OTTHUMP00000222779
• UniProt Protein Name: Parathyroid hormone
• Protein Family: Protein sprouty
• UniProt Entry Name: Q9BXY1_HUMAN

NCBI Description

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM]

Uniprot Description

SPRY4: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Belongs to the sprouty family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: Golgi apparatus; focal adhesion; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of MAP kinase activity; multicellular organismal development

Disease: Hypogonadotropic Hypogonadism 17 With Or Without Anosmia