Rabbit anti-Human, Mouse SPRN Polyclonal Antibody | anti-SPRN antibody

SPRN, CT (SPRN, SHO, Shadow of prion protein) (Biotin)

Cat. Num. AAA6350819

• Gene Names: SPRN; SHO; SHADOO; bA108K14.1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPRN; Polyclonal Antibody; CT (SPRN; SHO; Shadow of prion protein) (Biotin); Shadow of prion protein; anti-SPRN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SPRN antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SPRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83~113 amino acids from the C-terminal region of human SPRN.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SPRN Antibody (C-term) in mouse brain tissue lysates (35ug/lane). SPRN (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SPRN Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SPRN antibody

SPRN has PrP(C)-like neuroprotective activity. SPRN may act as a modulator for the biological actions of normal and abnormal PrP.

Product Categories/Family for anti-SPRN antibody

Antibodies; Proteins

References

Beck, J.A., et al. J. Med. Genet. 45(12):813-817(2008). Van den Brulle, J., et al. BioTechniques 45(3):340-343(2008). Premzl, M., et al. Mol. Biol. Evol. 21(12):2210-2231(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 61676082
• NCBI GeneID: 503542
• NCBI Accession #: NP_001012526.2
• NCBI GenBank Nucleotide #: NM_001012508.3
• UniProt Accession #: Q5BIV9
• Molecular Weight: 14,522 Da
• NCBI Official Full Name: shadow of prion protein
• NCBI Official Synonym Full Names: shadow of prion protein homolog (zebrafish)
• NCBI Official Symbol: SPRN
• NCBI Official Synonym Symbols: SHO; SHADOO; bA108K14.1
• NCBI Protein Information: shadow of prion protein; protein shadoo; hypothetical protein BC004409
• UniProt Protein Name: Shadow of prion protein
• Protein Family: Shadow of prion protein
• UniProt Gene Name: SPRN
• UniProt Synonym Gene Names: SHO; Protein shadoo
• UniProt Entry Name: SPRN_HUMAN

Uniprot Description

SPRN: Prion-like protein that has PrP(C)-like neuroprotective activity. May act as a modulator for the biological actions of normal and abnormal PrP. Belongs to the SPRN family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: plasma membrane; nucleolus; cytosol; vesicle

Molecular Function: nucleic acid binding

Biological Process: protein import into nucleus