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Western Blot (WB) (Western blot analysis of POLR3B expression in Hela (A), HepG2 (B) whole cell lysates.)

Rabbit POLR3B Polyclonal Antibody | anti-POLR3B antibody

Anti-POLR3B Antibody

Gene Names
POLR3B; C128; HLD8; RPC2; INMAP
Reactivity
Human, Mouse, Rat, Bovine, Dog, Monkey, Pig, Rabbit, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
POLR3B; Polyclonal Antibody; Anti-POLR3B Antibody; DNA-directed RNA polymerase III subunit RPC2; RNA polymerase III subunit C2; C128; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; anti-POLR3B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Monkey, Pig, Rabbit, Zebrafish
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of POLR3B protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Sequence Length
1133
Applicable Applications for anti-POLR3B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR3B. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of POLR3B expression in Hela (A), HepG2 (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of POLR3B expression in Hela (A), HepG2 (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of POLR3B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of POLR3B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-POLR3B antibody
Rabbit polyclonal antibody to POLR3B

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
121,120 Da
NCBI Official Full Name
DNA-directed RNA polymerase III subunit RPC2
NCBI Official Synonym Full Names
polymerase (RNA) III subunit B
NCBI Official Symbol
POLR3B
NCBI Official Synonym Symbols
C128; HLD8; RPC2; INMAP
NCBI Protein Information
DNA-directed RNA polymerase III subunit RPC2
UniProt Protein Name
DNA-directed RNA polymerase III subunit RPC2
UniProt Gene Name
POLR3B
UniProt Synonym Gene Names
RNA polymerase III subunit C2
UniProt Entry Name
RPC2_HUMAN

NCBI Description

This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

POLR3B: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Defects in POLR3B are the cause of leukodystrophy, hypomyelinating, type 8, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD8). An autosomal recessive neurodegenerative disorder characterized by early childhood onset of cerebellar ataxia and mild intellectual disabilities associated with diffuse hypomyelination apparent on brain MRI. Variable features include oligodontia and/or hypogonadotropic hypogonadism. Belongs to the RNA polymerase beta chain family.

Protein type: Nucleotide Metabolism - pyrimidine; EC 2.7.7.6; Nucleotide Metabolism - purine; Transcription initiation complex; Transferase

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasm

Molecular Function: DNA binding; metal ion binding; ribonucleoside binding

Biological Process: defense response to virus; gene expression; innate immune response; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 7, With Or Without Oligodontia And/or Hypogonadotropic Hypogonadism; Leukodystrophy, Hypomyelinating, 8, With Or Without Oligodontia And/or Hypogonadotropic Hypogonadism

Research Articles on POLR3B

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Product Notes

The POLR3B polr3b (Catalog #AAA8242374) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-POLR3B Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Monkey, Pig, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's POLR3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the POLR3B polr3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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