Western Blot (WB)
(Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit anti-Human Spred2 Polyclonal Antibody | anti-SPRED2 antibody
Spred2 Antibody
Gene Names
SPRED2; Spred-2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Spred2 Antibody is affinity chromatography purified via peptide column.
Synonyms
Spred2; Polyclonal Antibody; Spred2 Antibody; Spred-2; Sprouty-related; EVH1 domain-containing protein 2; sprouty-related; EVH1 domain containing 2; anti-SPRED2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.
Purity/Purification
Spred2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
418
Applicable Applications for anti-SPRED2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 mug/mL.
Conjugate
Unconjugated
Immunogen
Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2.
Buffer
Spred2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Spred2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.)
Related Product Information for anti-SPRED2 antibody
Spred2 Antibody: Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,033 Da
NCBI Official Full Name
sprouty-related, EVH1 domain-containing protein 2 isoform a
NCBI Official Synonym Full Names
sprouty-related, EVH1 domain containing 2
NCBI Official Symbol
SPRED2
NCBI Official Synonym Symbols
Spred-2
NCBI Protein Information
sprouty-related, EVH1 domain-containing protein 2; sprouty protein with EVH-1 domain 2, related sequence
UniProt Protein Name
Sprouty-related, EVH1 domain-containing protein 2
Protein Family
UniProt Gene Name
SPRED2
UniProt Synonym Gene Names
Spred-2
UniProt Entry Name
SPRE2_HUMAN
NCBI Description
SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]
Uniprot Description
SPRED2: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p14
Cellular Component: nucleoplasm; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase inhibitor activity; protein binding; stem cell factor receptor binding; protein kinase binding
Biological Process: multicellular organismal development; positive regulation of DNA damage response, signal transduction by p53 class mediator; inactivation of MAPK activity
Research Articles on SPRED2
Similar Products
Product Notes
The SPRED2 spred2 (Catalog #AAA150328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Spred2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Spred2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 mug/mL. Researchers should empirically determine the suitability of the SPRED2 spred2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Spred2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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