Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Rabbit anti-Human Granzyme M (GZMM) Polyclonal Antibody | anti-GZMM antibody
FITC-linked Antibody to Granzyme M (GZMM)
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity Chromatography.
Synonyms
Granzyme M (GZMM); Polyclonal Antibody; FITC-linked Antibody to Granzyme M (GZMM); anti-GZMM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GZMM. It has been selected for its ability to recognize GZMM in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-IIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR KVTGRSA
Sequence Length
264
Applicable Applications for anti-GZMM antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Note: As fluorescence can photobleach when exposed to light, so the antibody must be protected from light.
Note: As fluorescence can photobleach when exposed to light, so the antibody must be protected from light.
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: GZMM knockout K562 cell lysate; Predicted MW: 28kd Observed MW: 25kd Primary Ab: 1ug/ml Rabbit Anti-Human GZMM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: GZMM knockout K562 cell lysate; Predicted MW: 28kd Observed MW: 25kd Primary Ab: 1ug/ml Rabbit Anti-Human GZMM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Product Categories/Family for anti-GZMM antibody
NCBI and Uniprot Product Information
Uniprot Description
GZMM: Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells. Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted
Cellular Component: membrane
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity
Biological Process: cell death; cytolysis; proteolysis
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Product Notes
The GZMM (Catalog #AAA2017488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FITC-linked Antibody to Granzyme M (GZMM) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Granzyme M (GZMM) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GZMM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-IIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR KVTGRSA. It is sometimes possible for the material contained within the vial of "Granzyme M (GZMM), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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