Rabbit Spred1 Polyclonal Antibody | anti-Spred1 antibody

Spred1 Antibody

Cat. Num. AAA9414598

• Gene Names: SPRED1; NFLS; hSpred1; spred-1; PPP1R147
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Spred1; Polyclonal Antibody; Spred1 Antibody; Sprouty-related EVH1 domain-containing protein 1; NFLS; anti-Spred1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 444

• Applicable Applications for anti-Spred1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide near the center of the human Spred1.
• Target Name: Spred1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 ug/mL.)

Related Product Information for anti-Spred1 antibody

The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).

Product Categories/Family for anti-Spred1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 22749221
• NCBI GeneID: 161742
• NCBI Accession #: NP_689807
• NCBI GenBank Nucleotide #: NM_152594.2
• Molecular Weight: 50,477 Da
• NCBI Official Full Name: sprouty-related, EVH1 domain-containing protein 1
• NCBI Official Synonym Full Names: sprouty-related, EVH1 domain containing 1
• NCBI Official Symbol: SPRED1
• NCBI Official Synonym Symbols: NFLS; hSpred1; spred-1; PPP1R147
• NCBI Protein Information: sprouty-related, EVH1 domain-containing protein 1
• UniProt Protein Name: Sprouty-related, EVH1 domain-containing protein 1
• Protein Family: Sprouty-related, EVH1 domain-containing protein
• UniProt Gene Name: SPRED1
• UniProt Synonym Gene Names: Spred-1; hSpred1
• UniProt Entry Name: SPRE1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]

Uniprot Description

SPRED1: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Defects in SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS). It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleoplasm; cytoplasm; caveola

Molecular Function: protein serine/threonine kinase inhibitor activity; protein binding; stem cell factor receptor binding; protein kinase binding; phosphatase binding

Biological Process: multicellular organismal development; positive regulation of DNA damage response, signal transduction by p53 class mediator; inactivation of MAPK activity

Disease: Legius Syndrome