Rabbit anti-Human SPIRE1 Polyclonal Antibody | anti-SPIRE1 antibody

SPIRE1, CT (SPIRE1, KIAA1135, SPIR1, Protein spire homolog 1) (Azide free) (HRP)

Cat. Num. AAA6350611

• Gene Names: SPIRE1; Spir-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPIRE1; Polyclonal Antibody; CT (SPIRE1; KIAA1135; SPIR1; Protein spire homolog 1) (Azide free) (HRP); Protein spire homolog 1; anti-SPIRE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 622

• Applicable Applications for anti-SPIRE1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SPIRE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 664-691 amino acids from the C-terminal region of human SPIRE1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPIRE1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SPIRE1 antibody detected the SPIRE1 protein (arrow).)

Related Product Information for anti-SPIRE1 antibody

Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM].

Product Categories/Family for anti-SPIRE1 antibody

Antibodies; Transport Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Pechlivanis, M., et al. J. Biol. Chem. 284(37):25324-25333(2009). Bosch, M., et al. Mol. Cell 28(4):555-568(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Kerkhoff, E., et al. Curr. Biol. 11(24):1963-1968(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 191250775
• NCBI GeneID: 56907
• NCBI Accession #: NP_001122099.1; NP_001122098.1
• NCBI GenBank Nucleotide #: NP_001122099.1
• UniProt Accession #: Q08AE8
• NCBI Official Full Name: protein spire homolog 1 isoform c
• NCBI Official Synonym Full Names: spire type actin nucleation factor 1
• NCBI Official Symbol: SPIRE1
• NCBI Official Synonym Symbols: Spir-1
• NCBI Protein Information: protein spire homolog 1
• UniProt Protein Name: Protein spire homolog 1
• Protein Family: Protein spire
• UniProt Gene Name: SPIRE1
• UniProt Synonym Gene Names: KIAA1135; SPIR1; Spir-1
• UniProt Entry Name: SPIR1_HUMAN

NCBI Description

Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM, Mar 2008]

Uniprot Description

SPIRE1: Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport. Belongs to the spire family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18p11.21

Cellular Component: Golgi apparatus; cytoplasmic vesicle membrane; cytoskeleton; perinuclear region of cytoplasm; plasma membrane; cell cortex; cleavage furrow

Molecular Function: actin binding

Biological Process: vesicle-mediated transport; establishment of meiotic spindle localization; protein transport; intracellular transport; polar body extrusion after meiotic divisions; actin cytoskeleton organization and biogenesis; Golgi vesicle transport; actin nucleation