Rabbit SPHK1 Polyclonal Antibody | anti-SPHK1 antibody

SPHK1, ID (SPHK1, SPHK, SPK, Sphingosine kinase 1) (Azide free) (HRP)

Cat. Num. AAA6350545

• Gene Names: SPHK1; SPHK
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SPHK1; Polyclonal Antibody; ID (SPHK1; SPHK; SPK; Sphingosine kinase 1) (Azide free) (HRP); Sphingosine kinase 1; anti-SPHK1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SPHK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294~323 amino acids from the central region of human SPHK1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-SPHK1 Pab is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.)

Western Blot (WB)

(Western blot analysis of anti-SPHK1 Antibody (Center) in 293 cell line lysates (35ug/lane). SPHK1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-SPHK1 antibody

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Product Categories/Family for anti-SPHK1 antibody

Antibodies; Protein Kinases

References

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000). Melendez, A.J., et al., Gene 251(1):19-26 (2000). Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 17369329
• NCBI GeneID: 8877
• UniProt Accession #: Q9NYA1; Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3
• Molecular Weight: 384
• NCBI Official Full Name: Sphingosine kinase 1
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1; SK 1; SPK 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Synonym Gene Names: SPHK; SPK; SK 1; SPK 1
• UniProt Entry Name: SPHK1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 2.7.1.91; Kinase, lipid

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: synaptic vesicle; axon; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: D-erythro-sphingosine kinase activity; calmodulin binding; protein binding; DNA binding; protein phosphatase 2A binding; sphinganine kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein folding; positive regulation of NF-kappaB import into nucleus; female pregnancy; positive regulation of mitotic cell cycle; signal transduction; positive regulation of neurotransmitter secretion; activation of NF-kappaB transcription factor; 'de novo' posttranslational protein folding; response to magnesium ion; positive regulation of fibroblast proliferation; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of smooth muscle contraction; inflammatory response; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; cyclooxygenase pathway; calcium-mediated signaling; sphingosine metabolic process; cellular response to starvation; lipid phosphorylation; positive regulation of cell growth; response to ATP; positive regulation of angiogenesis; cellular protein metabolic process; positive regulation of protein ubiquitination; brain development; positive regulation of protein amino acid phosphorylation; response to progesterone stimulus; vascular endothelial growth factor receptor signaling pathway; sphingoid catabolic process; negative regulation of apoptosis; positive regulation of cell migration; response to amine stimulus