Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Dog Tumor Necrosis Factor Beta (TNFb) Polyclonal Antibody | anti-TNFb antibody
Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
Gene Names
LTA; TNFB; TNFSF1; TNLG1E; LT-alpha; TNF-beta
Reactivity
Dog
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Tumor Necrosis Factor Beta (TNFb); Polyclonal Antibody; Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb); anti-TNFb antibody
Host
Rabbit
Reactivity
Dog
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TNFb. It has been selected for its ability to recognize TNFb in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-LIGDP SIQNSLRWRA NTDRAFLRHG FSLSNNSLLV PTSGLYFVYS QVVFSGEGCF PKATPTPLYL AHEVQLFSSQ YPFHVPLLSA QKSVCPGPQG PWVRSVYQGA VFLLTQGDQL STHTD
MGHHHHHHSGSEF-LIGDP SIQNSLRWRA NTDRAFLRHG FSLSNNSLLV PTSGLYFVYS QVVFSGEGCF PKATPTPLYL AHEVQLFSSQ YPFHVPLLSA QKSVCPGPQG PWVRSVYQGA VFLLTQGDQL STHTD
Sequence Length
205
Applicable Applications for anti-TNFb antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TNFb (Leu66~Asp185) expressed in E.coli.
Cross Reactivity
Dog
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038545
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,033 Da
NCBI Official Full Name
lymphotoxin-alpha
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
TNFB; TNFSF1; TNLG1E; LT-alpha; TNF-beta
NCBI Protein Information
lymphotoxin-alpha
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Synonym Gene Names
TNFB; TNFSF1; LT-alpha
Uniprot Description
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo ().
Similar Products
Product Notes
The TNFb lta (Catalog #AAA2001372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Beta (TNFb) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TNFb lta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-LIGDP SIQNSLRWRA NTDRAFLRHG FSLSNNSLLV PTSGLYFVYS QVVFSGEGCF PKATPTPLYL AHEVQLFSSQ YPFHVPLLSA QKSVCPGPQG PWVRSVYQGA VFLLTQGDQL STHTD. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Beta (TNFb), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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