Western Blot (WB)
(Western Blot; Sample: Mouse Testis lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Sp17 AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
Rabbit Sperm Protein 17 Polyclonal Antibody | anti-Sp17 antibody
Sperm Protein 17 (Sp17) Polyclonal Antibody
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Mouse Testis lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Sp17 AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
Western Blot (WB)
(Western Blot; Sample: Mouse Testis lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Sp17 AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
Western Blot (WB)
(Western Blot: Sample: Recombinant Sp17, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Sp17, Human.)
Western Blot (WB)
(Western Blot; Sample: Rat Testis lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Sp17 AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Rat Testis lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Sp17 AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]
Uniprot Description
SPA17: Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: cilium; cytoplasm; external side of plasma membrane
Molecular Function: protein binding
Biological Process: binding of sperm to zona pellucida; single fertilization; spermatogenesis
Research Articles on Sp17
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Product Notes
The Sp17 spa17 (Catalog #AAA2032684) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sperm Protein 17 (Sp17) Polyclonal Antibody reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Sperm Protein 17 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the Sp17 spa17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sperm Protein 17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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