Testing Data
(Image: Detection of Spectrin Beta-III in human hepatocytes)
Rabbit anti-Human Spectrin, beta 2 Polyclonal Antibody
Spectrin, beta 2 (Spectrin beta Chain Brain 2, Spectrin beta Non-erythrocytic 2, SPTBN2, Spectrin Non-erythroid beta Chain 2, Spinocerebellar Ataxia 5, SCA5)
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Spectrin; beta 2; Polyclonal Antibody; beta 2 (Spectrin beta Chain Brain 2; Spectrin beta Non-erythrocytic 2; SPTBN2; Spectrin Non-erythroid beta Chain 2; Spinocerebellar Ataxia 5; SCA5); Anti -Spectrin; anti-Spectrin; beta 2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Spectrin, beta 2. Species Sequence Homology: Mouse and rat - 100%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid.
Applicable Applications for anti-Spectrin, beta 2 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Dilution: Immunohistochemistry: 2.5-5ug/ml
Immunoprecipitation: 2-5ug/mg lysate
Immunoprecipitation: 2-5ug/mg lysate
Immunogen
The epitope recognized by this antibody maps to a region between residues 2150 and 2200 of human spectrin, beta, non-erythrocytic 2 (spinocerebellar ataxia 5) using the numbering given in entry NP_008877.1
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Image: Detection of Spectrin Beta-III in human hepatocytes)
Testing Data
(Image: Detection of Spectrin Beta-III in human hepatocytes)
Western Blot (WB)
(Image: Detection of Human Spectrin Beta III (SPTBN2/SCA5) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 used for IP at 3 mcg/mg lysate. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody NB110-58346, which recognizes a downstream epitope. Detection: Chemiluminescence)
Western Blot (WB)
(Image: Detection of Human Spectrin Beta III (SPTBN2/SCA5) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 used for IP at 3 mcg/mg lysate. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody NB110-58346, which recognizes a downstream epitope. Detection: Chemiluminescence)
Related Product Information for anti-Spectrin, beta 2 antibody
Mutations in spectrins are a previously unknown cause of ataxia and neurodegenerative disease that affect membrane proteins involved in glutamate signaling. Spectrin-beta IIs have been recognized as ataxia disease genes and their mutations cause spinocerebellar ataxia type 5 (SCA5).
Product Categories/Family for anti-Spectrin, beta 2 antibody
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Product Notes
The Spectrin, beta 2 (Catalog #AAA623232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Spectrin, beta 2 (Spectrin beta Chain Brain 2, Spectrin beta Non-erythrocytic 2, SPTBN2, Spectrin Non-erythroid beta Chain 2, Spinocerebellar Ataxia 5, SCA5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Spectrin, beta 2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC). Dilution: Immunohistochemistry: 2.5-5ug/ml Immunoprecipitation: 2-5ug/mg lysate. Researchers should empirically determine the suitability of the Spectrin, beta 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Spectrin, beta 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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