Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): A549 and lncap cell, human seminoma tissue Amount of lysate: 60ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 minutes)
Rabbit anti-Human SPATA20 Polyclonal Antibody | anti-SPATA20 antibody
SPATA20 Antibody
Gene Names
SPATA20; SSP411; Tisp78
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
SPATA20; Polyclonal Antibody; SPATA20 Antibody; SSP411; Tisp78; anti-SPATA20 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total SPATA20 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
2.8 mg/ml (varies by lot)
Sequence Length
786
Applicable Applications for anti-SPATA20 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:100-1:300
Immunohistochemistry: 1:100-1:300
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to a region derived from internal residues of human spermatogenesis associated 20
Target Name
SPATA20
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): A549 and lncap cell, human seminoma tissue Amount of lysate: 60ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 minutes)
Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): A549 and lncap cell, human seminoma tissue Amount of lysate: 60ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 minutes)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/70.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/70.)
Related Product Information for anti-SPATA20 antibody
The protein may play a role in fertility regulation. It were significantly upregulated in individual bile samples from Cholangiocarcinoma patients by Western blotting and Immunohistochemistry, relative to normal tissues.It acts as a potential serum biomarker for Cholangiocarcinoma.
Product Categories/Family for anti-SPATA20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,463 Da
NCBI Official Full Name
spermatogenesis-associated protein 20 isoform 2
NCBI Official Synonym Full Names
spermatogenesis associated 20
NCBI Official Symbol
SPATA20
NCBI Official Synonym Symbols
SSP411; Tisp78
NCBI Protein Information
spermatogenesis-associated protein 20
UniProt Protein Name
Spermatogenesis-associated protein 20
Protein Family
UniProt Gene Name
SPATA20
UniProt Synonym Gene Names
Ssp411
UniProt Entry Name
SPT20_HUMAN
Uniprot Description
SPATA20: May play a role in fertility regulation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: extracellular region
Molecular Function: catalytic activity
Biological Process: metabolic process; multicellular organismal development; spermatogenesis; cell differentiation
Research Articles on SPATA20
Similar Products
Product Notes
The SPATA20 spata20 (Catalog #AAA9401052) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPATA20 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPATA20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500-1:2000 Immunohistochemistry: 1:100-1:300. Researchers should empirically determine the suitability of the SPATA20 spata20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPATA20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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