Rabbit anti-Human, Mouse SPATA18 Polyclonal Antibody | anti-SPATA18 antibody

SPATA18, NT (SPATA18, MIEAP, Mitochondria-eating protein, Spermatogenesis-associated protein 18) (Biotin)

Cat. Num. AAA6350334

• Gene Names: SPATA18; Mieap; SPETEX1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPATA18; Polyclonal Antibody; NT (SPATA18; MIEAP; Mitochondria-eating protein; Spermatogenesis-associated protein 18) (Biotin); Spermatogenesis-associated protein 18; anti-SPATA18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SPATA18 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SPATA18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human SPATA18.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPATA18 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).)

Western Blot (WB)

(SPATA18 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).)

Immunohistochemistry (IHC)

(SPATA18 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPATA18 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SPATA18 antibody

Potential role in spermatogenesis, especially in cell differentiation from late elongate spematids to mature spermatozoa (By similarity).

Product Categories/Family for anti-SPATA18 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009). Kaindl, A.M., et al. Hum. Mutat. 26(3):279-280(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 21687119
• NCBI GeneID: 132671
• NCBI Accession #: NP_660306.1
• NCBI GenBank Nucleotide #: NM_145263.3
• UniProt Accession #: Q8TC71; Q8IY48; Q8N7D7; B4E2R0; E5RLK1
• Molecular Weight: 57,485 Da
• NCBI Official Full Name: mitochondria-eating protein isoform 1
• NCBI Official Synonym Full Names: spermatogenesis associated 18
• NCBI Official Symbol: SPATA18
• NCBI Official Synonym Symbols: Mieap; SPETEX1
• NCBI Protein Information: mitochondria-eating protein; spermatogenesis associated 18 homolog; spermatogenesis-associated protein 18
• UniProt Protein Name: Mitochondria-eating protein
• Protein Family: Mitochondria-eating protein
• UniProt Gene Name: SPATA18
• UniProt Synonym Gene Names: MIEAP
• UniProt Entry Name: MIEAP_HUMAN

Uniprot Description

SPATA18: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Belongs to the MIEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: protein binding

Biological Process: response to DNA damage stimulus