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Testing Data (Gel: 12%SDS-PAGE Lysates (from left to right): Human liver cancer tissue Amount of lysate: 50ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)

Rabbit anti-Human SPATA16 Polyclonal Antibody | anti-SPATA16 antibody

SPATA16 Antibody

Gene Names
SPATA16; SPGF6; NYD-SP12
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
SPATA16; Polyclonal Antibody; SPATA16 Antibody; SPGF6; NYD-SP12; anti-SPATA16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total SPATA16 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
0.8 mg/ml (varies by lot)
Sequence Length
569
Applicable Applications for anti-SPATA16 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:15-1:50
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to residues near the C terminal of human Spermatogenesis associated 16
Target Name
SPATA16
Preparation and Storage
Store at -20 degree C

Testing Data

(Gel: 12%SDS-PAGE Lysates (from left to right): Human liver cancer tissue Amount of lysate: 50ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)

Testing Data (Gel: 12%SDS-PAGE Lysates (from left to right): Human liver cancer tissue Amount of lysate: 50ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/25.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/25.)
Related Product Information for anti-SPATA16 antibody
This gene encodes a testis-specific protein that belongs to the tetratricopeptide repeat-like superfamily. The encoded protein localizes to the Golgi apparatus and may play a role in spermatogenesis. The SPATA16 protein contains a region of homology to the mouse male enhanced antigen-2, a SCOP domain belonging to the tetratricopeptide repeat-like superfamily, 3 glycosylation sites, and several phosphorylation sites. The mouse and human proteins share 77% sequence identity. Northern blot analysis of multiple human tissues detected a strong signal in testis and weak expression in pancreas and kidney. Laser confocal microscopy localized a GFP-SPATA16 fusion protein specifically to the Golgi apparatus.
Product Categories/Family for anti-SPATA16 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,263 Da
NCBI Official Full Name
spermatogenesis-associated protein 16
NCBI Official Synonym Full Names
spermatogenesis associated 16
NCBI Official Symbol
SPATA16
NCBI Official Synonym Symbols
SPGF6; NYD-SP12
NCBI Protein Information
spermatogenesis-associated protein 16
UniProt Protein Name
Spermatogenesis-associated protein 16
UniProt Gene Name
SPATA16
UniProt Entry Name
SPT16_HUMAN

NCBI Description

This gene encodes a testis-specific protein that belongs to the tetratricopeptide repeat-like superfamily. The encoded protein localizes to the Golgi apparatus and may play a role in spermatogenesis. [provided by RefSeq, May 2010]

Uniprot Description

SPATA16: Involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. Defects in SPATA16 are the cause of spermatogenic failure type 6 (SPGF6); also known as round-headed spermatozoa. An infertility disorder caused by spermatogenesis defects. The most prominent feature is the malformation of the acrosome, which can be totally absent in most severe cases. Additional features are an abnormal nuclear shape and abnormal arrangement of the mitochondria of the spermatozoon. An autosomal recessive variation of SPATA16 has been shown to be responsible for the disease in a consanguineous family with members homozygous for the variation. Belongs to the SPATA16 family.

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: Golgi apparatus

Biological Process: multicellular organismal development; spermatogenesis; cell differentiation

Disease: Spermatogenic Failure 6

Research Articles on SPATA16

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Product Notes

The SPATA16 spata16 (Catalog #AAA9401394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPATA16 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPATA16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:15-1:50. Researchers should empirically determine the suitability of the SPATA16 spata16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPATA16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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