Rabbit SPARC Polyclonal Antibody | anti-SPARC antibody

SPARC Antibody

Cat. Num. AAA9412513

• Gene Names: SPARC; ON; BM-40
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: SPARC; Polyclonal Antibody; SPARC Antibody; ON; anti-SPARC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SPARC protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 303

• Applicable Applications for anti-SPARC antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human SPARC.
• Target Name: SPARC
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SPARC antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney using SPARC antibody at dilution of 1:100 (200x lens).)

Related Product Information for anti-SPARC antibody

SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin (1). Members in this class of glycoproteins are involved in tissue renewal, tissue remodeling, and embryonic development and work by exerting counter-adhesive and antiproliferative effects that lead to changes in cell shape, disruption of cell adhesion, and inhibition of the cell cycle (2). SPARC is expressed at high levels in bone tissue but is widely distributed in many other tissues and cell types (3), and is known to be associated with tissues undergoing morphogenesis, angiogenesis, mineralization, and other pathological responses to injury and tumorigenesis (4,5). SPARC has also been linked with obesity and diabetes (6).

Product Categories/Family for anti-SPARC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507171
• NCBI GeneID: 6678
• NCBI Accession #: NP_003109.1
• NCBI GenBank Nucleotide #: NM_003118.3
• UniProt Accession #: P09486; Q6IBK4; D3DQH9
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: SPARC isoform 1
• NCBI Official Synonym Full Names: secreted protein, acidic, cysteine-rich (osteonectin)
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON; BM-40
• NCBI Protein Information: SPARC
• UniProt Protein Name: SPARC
• Protein Family: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: receptor-mediated endocytosis; response to peptide hormone stimulus; response to gravity; platelet activation; ossification; extracellular matrix organization and biogenesis; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii