Rabbit anti-Human SPAG8 Polyclonal Antibody | anti-SPAG8 antibody
SPAG8 Antibody
Gene Names
SPAG8; SMP1; BS-84; CT142; HSD-1; SPAG3; CILD28; hSMP-1
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SPAG8; Polyclonal Antibody; SPAG8 Antibody; BS 84; CILD28; HSD 1; HSD-1; HSD1; hSMP1; MGC26201; MH SPAG8; OTTHUMP00000021352; OTTMUSP00000032209; SMP-1; SMP1; SPAG3; SPAG8_HUMAN; Sperm associated antigen 8; Sperm associated antigen 8 like protein; Sperm membrane protein 1; Sperm membrane protein BS-84; Sperm-associated antigen 8; Testicular tissue protein Li 177; anti-SPAG8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
SPAG8 Antibody detects endogenous levels of SPAG8.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SPAG8 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human SPAG8.
Conjugation
Unconjugated
Similarity
Belongs to the SPAG8 family.
Subunit Structure
Interacts with FHL5 (via second LIM domain) (By similarity). Interacts with RANBP9.
Tissue Specificity
Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine.
Function
Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (By similarity). Involved in the acrosome reaction and in binding of sperm to the zona pellucida (By similarity). Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. May play a role in fertility and microtubule formation through interaction with RANBP9.
Note
In mature sperm cells, detected in the acrosomal region of the head and in the middle piece of the tail (By similarity). Localized to the nucleus and cytoplasm of spermatocytes and round spermatids while, in elongating spermatids, expressed in the cytoplasm but not in the nucleus (By similarity). During the cell cycle, localized on the microtubule-organizing center (MTOC) during prophase. In metaphase, extends along spindle microtubules. In anaphase, detected on the astral microtubules and mid-zone. In telophase, remains at the mid-zone. After cytokinesis, returns to the MTOC (PubMed:19548270).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-SPAG8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,139 Da
NCBI Official Full Name
sperm-associated antigen 8 isoform 2
NCBI Official Synonym Full Names
sperm associated antigen 8
NCBI Official Symbol
SPAG8
NCBI Official Synonym Symbols
SMP1; BS-84; CT142; HSD-1; SPAG3; CILD28; hSMP-1
NCBI Protein Information
sperm-associated antigen 8; sperm membrane protein 1; sperm membrane protein BS-84
UniProt Protein Name
Sperm-associated antigen 8
Protein Family
UniProt Gene Name
SPAG8
UniProt Synonym Gene Names
SMP-1
UniProt Entry Name
SPAG8_HUMAN
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Product Notes
The SPAG8 spag8 (Catalog #AAA9630616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPAG8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPAG8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SPAG8 spag8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPAG8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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