Western Blot (WB)
(Western blot analysis of Mouse urinary bladder tissue lysate using SPAG4 Polyclonal Antibody at dilution of 1:400)
Rabbit anti-Human, Mouse SPAG4 Polyclonal Antibody | anti-SPAG4 antibody
SPAG4 Polyclonal Antibody
Gene Names
SPAG4; SUN4; CT127
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purification
Synonyms
SPAG4; Polyclonal Antibody; SPAG4 Polyclonal Antibody; acrosomal protein ACR55; Cancer/testis antigen 127; CT127; OTTHUMP00000030768; OTTHUMP00000204168; Outer dense fiber-associated protein SPAG4; Sad1 and UNC84 domain containing 4; Spag4; Sperm associated antigen 4; Sperm tail protein; Sperm-associated antigen 4 protein; SUN domain-containing protein 4; SUN4; anti-SPAG4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Concentration
1.02mg/mL (varies by lot)
Sequence Length
437
Applicable Applications for anti-SPAG4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:30-1:150
ELISA: 1:5000-1:10000
IHC: 1:30-1:150
ELISA: 1:5000-1:10000
Immunogen
Synthetic peptide of human SPAG4
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Mouse urinary bladder tissue lysate using SPAG4 Polyclonal Antibody at dilution of 1:400)
Western Blot (WB)
(Western blot analysis of Mouse urinary bladder tissue lysate using SPAG4 Polyclonal Antibody at dilution of 1:400)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human brain tissue using SPAG4 Polyclonal Antibody at dilution of 1:35(×200))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human brain tissue using SPAG4 Polyclonal Antibody at dilution of 1:35(×200))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SPAG4 Polyclonal Antibody at dilution of 1:35(×200))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SPAG4 Polyclonal Antibody at dilution of 1:35(×200))
Related Product Information for anti-SPAG4 antibody
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.
Product Categories/Family for anti-SPAG4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48 kDa
NCBI Official Full Name
sperm-associated antigen 4 protein isoform 1
NCBI Official Synonym Full Names
sperm associated antigen 4
NCBI Official Symbol
SPAG4
NCBI Official Synonym Symbols
SUN4; CT127
NCBI Protein Information
sperm-associated antigen 4 protein
UniProt Protein Name
Sperm-associated antigen 4 protein
Protein Family
UniProt Gene Name
SPAG4
UniProt Synonym Gene Names
SUN4
UniProt Entry Name
SPAG4_HUMAN
NCBI Description
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Research Articles on SPAG4
Similar Products
Product Notes
The SPAG4 spag4 (Catalog #AAA2563174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPAG4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPAG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:30-1:150 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the SPAG4 spag4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPAG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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