Rabbit SP1 Polyclonal Antibody | anti-SP1 antibody

SP1 (Phospho-Thr453) Antibody

Cat. Num. AAA9502547

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: SP1; Polyclonal Antibody; SP1 (Phospho-Thr453) Antibody; anti-SP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: SP1 (Phospho-Thr453) Antibody detects endogenous levels of SP1 only when phosphorylated at Thr453.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 737

• Applicable Applications for anti-SP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:T453; M:T455; R:T456
• Immunogen: The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr453.
• Immunogen Range: 421-470
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from A549 cells, using SP1 (Phospho-Thr453) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 352962149
• NCBI GeneID: 6667
• NCBI Accession #: NP_001238754.1
• NCBI GenBank Nucleotide #: NM_001251825.1
• UniProt Accession #: P08047; Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7; E4Z9M7; G5E9M8
• Molecular Weight: 80 kDa
• NCBI Official Full Name: transcription factor Sp1 isoform c
• NCBI Official Synonym Full Names: Sp1 transcription factor
• NCBI Official Symbol: SP1
• NCBI Protein Information: transcription factor Sp1
• UniProt Protein Name: Transcription factor Sp1
• Protein Family: Sp110 nuclear body protein
• UniProt Gene Name: SP1
• UniProt Synonym Gene Names: TSFP1

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.