Rabbit anti-Human, Mouse SOX9 Polyclonal Antibody | anti-SOX9 antibody

Rabbit Polyclonal (IgG) to Human SOX9

Cat. Num. AAA244975

• Gene Names: SOX9; CMD1; SRA1; CMPD1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SOX9; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SOX9; Anti-SOX9 Antibody (aa147-196) IHC-plus; CMD1; CMPD1; SRY-related HMG-box; gene 9; Transcription factor SOX-9; Human SOX9; anti-SOX9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SOX9 Antibody detects endogenous levels of total SOX9 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-SOX9 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SOX9 (aa147-196).
• Immunogen: SOX9 antibody was raised against synthetic peptide from human SOX9 (aa147-196).
• Antigen Modification: aa147-196
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SOX9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using SOX9 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from 293 cells, treated with PBS 60 minutes, using SOX9 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-SOX9 antibody

The SOX9 protein recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Product Categories/Family for anti-SOX9 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 4557853
• NCBI GeneID: 6662
• NCBI Accession #: NP_000337.1
• NCBI GenBank Nucleotide #: NM_000346.3
• UniProt Accession #: P48436; Q53Y80
• Molecular Weight: 56,137 Da
• NCBI Official Full Name: transcription factor SOX-9
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 9
• NCBI Official Symbol: SOX9
• NCBI Official Synonym Symbols: CMD1; SRA1; CMPD1
• NCBI Protein Information: transcription factor SOX-9; SRY-related HMG-box, gene 9; SRY (sex-determining region Y)-box 9 protein
• UniProt Protein Name: Transcription factor SOX-9
• Protein Family: Transcription factor
• UniProt Gene Name: SOX9
• UniProt Entry Name: SOX9_HUMAN

NCBI Description

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX9: Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Defects in SOX9 are the cause of campomelic dysplasia (CMD1). CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. Defects in SOX9 are the cause of 46,XX sex reversal type 2 (SRXX2). SRXX2 is a condition in which male gonads develop in a genetic female (female to male sex reversal).

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: nucleoplasm; protein complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; beta-catenin binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; protein kinase activity

Biological Process: prostate gland development; extracellular matrix organization and biogenesis; somatic stem cell maintenance; astrocyte fate commitment; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; negative regulation of epithelial cell differentiation; notochord development; protein amino acid phosphorylation; regulation of apoptosis; negative regulation of bone mineralization; cell-cell adhesion; hair follicle development; positive regulation of mesenchymal cell proliferation; tissue homeostasis; negative regulation of ossification; oligodendrocyte differentiation; positive regulation of epithelial cell differentiation; protein complex assembly; cartilage condensation; negative regulation of photoreceptor cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; nucleosome assembly; positive regulation of chondrocyte differentiation; retina development in camera-type eye; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; transcription from RNA polymerase II promoter; neural crest cell development; Sertoli cell differentiation; cell fate specification; negative regulation of immune system process; signal transduction; cAMP-mediated signaling; mammary gland development; positive regulation of cell proliferation; protein kinase B signaling cascade; otic vesicle formation; skeletal development; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; ossification; male gonad development; cytoskeleton organization and biogenesis; endocrine pancreas development; Sertoli cell development; regulation of cell proliferation; male germ-line sex determination; chromatin remodeling; limb bud formation; ureteric bud branching; cartilage development; epithelial to mesenchymal transition; spermatogenesis; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation

Disease: Campomelic Dysplasia; 46,xy Sex Reversal 10