Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using DHPS antibody.)
Rabbit anti-Human, Mouse DHPS Polyclonal Antibody | anti-DHPS antibody
DHPS Antibody
IF/ICC 1:100-1:500
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using DHPS antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using DHPS antibody.)
Immunofluorescene (IF)
(MBS9605443 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(MBS9605443) and mouse anti-betatubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605443 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(MBS9605443) and mouse anti-betatubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]
Uniprot Description
Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
Research Articles on DHPS
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Product Notes
The DHPS dhps (Catalog #AAA9605443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHPS Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHPS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB 1:500-1:1000 IF/ICC 1:100-1:500 ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the DHPS dhps for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHPS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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