Rabbit anti-Human Sox17 Polyclonal Antibody | anti-Sox17 antibody

Sox17 (Transcription Factor SOX-17, Sox17, Sox-17) (MaxLight 650)

Cat. Num. AAA6459393

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Sox17; Polyclonal Antibody; Sox17 (Transcription Factor SOX-17; Sox-17) (MaxLight 650); anti-Sox17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Sox17. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-Sox17 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide corresponding to aa376-409 at the C-terminal region of mouse Sox17.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-Sox17 antibody

Antibodies; Transcription Factors

References

Kanai Y., et al. J. Cell Biol. 133:667-681(1996). Carninci P., et al. Science 309:1559-1563(2005). Layfield R., et al. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases. Kanai-Azuma M., et al. Development 129:2367-2379(2002). Kim I., et al. Cell 130:470-483(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 575501715
• NCBI GeneID: 20671
• NCBI Accession #: NP_001276393.1
• NCBI GenBank Nucleotide #: NM_001289464.1
• UniProt Accession #: Q61473; Q61472; Q62248
• Molecular Weight: 30,782 Da
• NCBI Official Full Name: transcription factor SOX-17 isoform a
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 17
• NCBI Official Symbol: Sox17
• NCBI Protein Information: transcription factor SOX-17
• UniProt Protein Name: Transcription factor SOX-17
• Protein Family: Transcription factor
• UniProt Gene Name: Sox17
• UniProt Synonym Gene Names: Sox-17
• UniProt Entry Name: SOX17_MOUSE

NCBI Description

This gene encodes a member of the Sox (Sry-related high mobility group box) family of transcription factors involved in the regulation of embryonic development. The encoded protein plays a role in the determination of cell fate and in maintaining cell identity. This gene regulates tumor angiogenesis and tumor progression. Mutations in the human gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

SOX17: Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'- AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells. Defects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3). VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.

Protein type: Transcription regulation; DNA-binding

Cellular Component: transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; beta-catenin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; endodermal cell fate determination; negative regulation of Wnt receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of embryonic development; stem cell fate specification; gastrulation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; rostrocaudal neural tube patterning; regulation of transcription, DNA-dependent; negative regulation of mesodermal cell fate specification; regulation of cell differentiation; embryonic foregut morphogenesis; inner cell mass cellular morphogenesis; heart looping; angiogenesis; vasculogenesis; cell migration involved in gastrulation; Wnt receptor signaling pathway; transcription, DNA-dependent; protein stabilization; protein destabilization; positive regulation of skeletal muscle development; stem cell differentiation; mRNA transcription from RNA polymerase II promoter; regulation of mesodermal cell fate specification; endoderm formation; positive regulation of protein catabolic process; embryonic heart tube development; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; negative regulation of cell growth; positive regulation of cell differentiation; endoderm development