Rabbit Sodium Potassium ATPase alpha-1 Polyclonal Antibody | anti-ATP1A1 antibody

Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Antibody

Cat. Num. AAA9419697

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• Synonyms: Sodium Potassium ATPase alpha-1; Polyclonal Antibody; Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Antibody; Sodium/potassium-transporting ATPase subunit alpha-1 (Na (+)/K (+) ATPase alpha-1 subunit) (EC 3.6.3.9) (Sodium pump subunit alpha-1); Sodium Potassium ATPase alpha-1 (Phospho-Tyr260); anti-ATP1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous Sodium Potassium ATPase alpha-1 when Phospho occurs at Tyr260)
• Purity/Purification: Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-ATP1A1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Synthetic peptide from human protein at AA range: 230-290
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000)

Product Categories/Family for anti-ATP1A1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z2T5; B7Z3U6; F5H3A1
• Molecular Weight: 115 kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 1
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: endoplasmic reticulum; Golgi apparatus; integral to membrane; membrane; plasma membrane; protein complex; sarcolemma; sodium:potassium-exchanging ATPase complex

Molecular Function: ATP binding; chaperone binding; potassium ion binding; protein binding; sodium ion binding; sodium:potassium-exchanging ATPase activity

Biological Process: cellular potassium ion homeostasis; cellular sodium ion homeostasis; potassium ion import; regulation of sodium ion transport