Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Intended Uses
This ST1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ST1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ST1 elisa kit
Principle of the assay: ST1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ST1 antibody and an ST1-HRP conjugate. The assay sample and buffer are incubated together with ST1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ST1 concentration since ST1 from samples and ST1-HRP conjugate compete for the anti-ST1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ST1 from the sample, fewer sites are left to bind ST1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GenBank Nucleotide #
Molecular Weight
53,977 Da
NCBI Official Full Name
stromelysin-1 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 3 (stromelysin 1, progelatinase)
NCBI Official Symbol
MMP3
NCBI Official Synonym Symbols
SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
NCBI Protein Information
stromelysin-1; transin-1; proteoglycanase; matrix metalloproteinase-3; matrix metalloproteinase 3 (stromelysin 1, progelatinase)
UniProt Protein Name
Stromelysin-1
UniProt Synonym Gene Names
STMY1; SL-1; MMP-3
UniProt Entry Name
MMP3_HUMAN
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family.
Protein type: Protease; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.17; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: extracellular space; proteinaceous extracellular matrix; mitochondrion; extracellular region; cytosol
Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding
Biological Process: collagen catabolic process; positive regulation of protein oligomerization; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration
Disease: Coronary Heart Disease, Susceptibility To, 6
Research Articles on ST1
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Product Notes
The Bovine ST1 mmp3 (Catalog #
AAA745170) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745170 ELISA Kit recognizes Bovine ST1.
It is sometimes possible for the material contained within the vial of
"Stromelysin-1, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
cannot be held
responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
any time and without notice. It is the responsibility of the customer to inform AAA Biotech
of any product performance
issues observed or experienced within 30 days of receipt of said product. To see additional
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