Goat anti-Mouse, Rat SODD Polyclonal Antibody | anti-BAG4 antibody

SODD (Silencer of Death Domains)

Cat. Num. AAA612502

• Gene Names: BAG4; SODD; BAG-4; DKFZp586O2022
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SODD; Polyclonal Antibody; SODD (Silencer of Death Domains); Anti -SODD (Silencer of Death Domains); anti-BAG4 antibody

• Host: Goat
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous mouse and rat SODD/BAG4 in Western Blots.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose.

• Applicable Applications for anti-BAG4 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1.0ug/ml
• Immunogen: E. coli-derived recombinant mouse SODD/BAG4 (aa1-457)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-BAG4 antibody

Tumor necrosis factor receptor-1 (TNF-R1) and other TNF receptor superfamily members, such as DR3, contain intracellular death domains (DD) and are capable of initiating apoptosis when activated by their ligands. Silencer of Death Domains (SODD) was identified as being involved in the cellular mechanism to protect against ligand-independent signaling by TNF-R1 and other DD receptors. SODD, also known as Bcl-2-Associated Athanogene 4 (BAG4), is a 457 amino acid (aa), anti-apoptotic protein that functions through interactions with a variety of proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors, and members of the heat shock protein 70 kD family. SODD is a ubiquitously expressed, cytoplasmic protein that contains a C terminal BAG domain that can bind and inhibit the chaperone activity of Hsc70/Hsp70. The association of SODD with the DD of TNF-R1 prevents constitutive activation of the TNF-R1 signaling pathway. Binding of TNF to TNF-R1 releases SODD and permits adapter molecules such as TRADD to associate with TNF-R1 leading to the activation of TNF signaling pathways such as apoptosis and NFkB activation.

Product Categories/Family for anti-BAG4 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4160014
• NCBI GeneID: 9530
• UniProt Accession #: O95429; O95818
• Molecular Weight: 49,594 Da
• NCBI Official Full Name: silencer of death domains
• NCBI Official Synonym Full Names: BCL2-associated athanogene 4
• NCBI Official Symbol: BAG4
• NCBI Official Synonym Symbols: SODD; BAG-4; DKFZp586O2022
• NCBI Protein Information: BAG family molecular chaperone regulator 4; OTTHUMP00000225745; OTTHUMP00000225746; silencer of death domains; bcl-2-associated athanogene 4; BAG-family molecular chaperone regulator-4
• UniProt Protein Name: BAG family molecular chaperone regulator 4
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG4
• UniProt Synonym Gene Names: SODD
• UniProt Entry Name: BAG4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Uniprot Description

BAG4: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Prevents constitutive TNFRSF1A signaling. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: plasma membrane; cytosol; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; protein folding; positive regulation of actin filament polymerization; protein heterooligomerization; positive regulation of stress fiber formation; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis